Standard Curve (Sample)
Mouse UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit | UGCG elisa kit
Mouse UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit
Gene Names
UGCG; GCS; GLCT1
Reactivity
Mouse
Synonyms
UDP Glucose Ceramide Glucosyltransferase (UGCG); N/A; Mouse UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit; UGCG elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of UGCG. No significant cross-reactivity or interference between UGCG and analogues was observed.
Sequence Length
394
Samples
Mouse tissue homogenates and other biological fluids
Detection Range
0.78 -50ng/mL
Sensitivity
The minimum detectable dose of mouse UGCG is typically less than 0.28ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UGCG were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UGCG were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intended Uses
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of UGCG in mouse tissue homogenates and other biological fluids.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for UGCG elisa kit
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UGCG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UGCG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UGCG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UGCG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,854 Da
NCBI Official Full Name
UDP-glucose ceramide glucosyltransferase
NCBI Official Synonym Full Names
UDP-glucose ceramide glucosyltransferase
NCBI Official Symbol
UGCG
NCBI Official Synonym Symbols
GCS; GLCT1
NCBI Protein Information
ceramide glucosyltransferase; GLCT-1; glucosylceramide synthase; UDP-glucose:N-acylsphingosine D-glucosyltransferase
UniProt Protein Name
Ceramide glucosyltransferase
UniProt Gene Name
UGCG
UniProt Synonym Gene Names
GCS
UniProt Entry Name
CEGT_HUMAN
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Product Notes
The Mouse UGCG ugcg (Catalog #AAA138064) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA138064 ELISA Kit recognizes Mouse UGCG. It is sometimes possible for the material contained within the vial of "UDP Glucose Ceramide Glucosyltransferase (UGCG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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