Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit | UGCG elisa kit

Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit

Cat. Num. AAA137545

• Gene Names: UGCG; GCS; GLCT1
• Reactivity: Rat
• Synonyms: UDP Glucose Ceramide Glucosyltransferase (UGCG); N/A; Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit; UGCG elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of UDP Glucose Ceramide Glucosyltransferase (UGCG); No significant cross-reactivity or interference between UDP Glucose Ceramide Glucosyltransferase (UGCG) and analogues was observed.
• Sequence Length: 394
• Samples: Tissue homogenates, Cell lysates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL The standard curve concentrations used for the ELISA's were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL
• Sensitivity: Typically less than 0.65ng/mL.

• Organism Species: Rattus norvegicus (Rat)
• ELISA Type: Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UDP Glucose Ceramide Glucosyltransferase (UGCG) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UDP Glucose Ceramide Glucosyltransferase (UGCG) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for UGCG elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucose Ceramide Glucosyltransferase (UGCG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucose Ceramide Glucosyltransferase (UGCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucose Ceramide Glucosyltransferase (UGCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP Glucose Ceramide Glucosyltransferase (UGCG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 24270822
• NCBI GeneID: 7357
• UniProt Accession #: Q16739; Q5T258
• Molecular Weight: 44,854 Da
• NCBI Official Full Name: UDP-glucose ceramide glucosyltransferase
• NCBI Official Synonym Full Names: UDP-glucose ceramide glucosyltransferase
• NCBI Official Symbol: UGCG
• NCBI Official Synonym Symbols: GCS; GLCT1
• NCBI Protein Information: ceramide glucosyltransferase; GLCT-1; glucosylceramide synthase; UDP-glucose:N-acylsphingosine D-glucosyltransferase
• UniProt Protein Name: Ceramide glucosyltransferase
• UniProt Gene Name: UGCG
• UniProt Synonym Gene Names: GCS
• UniProt Entry Name: CEGT_HUMAN