Mouse VEGF-B PicoKine ELISA Kit | Vegfb elisa kit

Mouse VEGF-B PicoKine ELISA Kit

Cat. Num. AAA46173

• Gene Names: Vegfb; Vrf; VEGF-B
• Reactivity: Mouse
• Synonyms: VEGF-B PicoKine; N/A; Mouse VEGF-B PicoKine ELISA Kit; Vascular endothelial growth factor B; VEGF B; VEGF related factor; VEGF-B; VEGF-related factor; Vegfb; VEGFB_HUMAN; VEGFL; VRF; ; vascular endothelial growth factor B; Vegfb elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse VEGF-B
• Sequence Length: 188
• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <2pg/ml
• Sample: For quantitative detection of mouse VEGF-B in cell culture supernates, serum and plasma(heparin).

• Immunogen: sf21,Q20-A207&Q20-R148
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse VEGF-B standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse VEGF-B standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|158|652|1309|197|763|1611; Standard deviation|6.95|33.25|74.61|14.58|54.94|124.05; CV(%)|4.4|5.1|5.7|7.4|7.2|7.7
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.006|0.122|0.245|0.441|0.743|1.101|1.748|2.241
• Anticoagulant: Heparin
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Mouse VEGF-B PicoKine ELISA Kit standard curve)

Related Product Information for Vegfb elisa kit

Principle of the assay: Mouse VEGF-B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards(sf21,Q20-A207 & Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGF-B amount of sample captured in plate.

Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

NCBI and Uniprot Product Information

• NCBI GI #: 297632418
• NCBI GeneID: 22340
• NCBI Accession #: NP_001172093.1
• NCBI GenBank Nucleotide #: NM_001185164.1
• UniProt Accession #: P49766; Q3UG04; Q5D0B1; Q64290
• Molecular Weight: 21,442 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform Vegf-b167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: Vegfb
• NCBI Official Synonym Symbols: Vrf; VEGF-B
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• UniProt Gene Name: Vegfb
• UniProt Synonym Gene Names: Vrf; VEGF-B; VRF
• UniProt Entry Name: VEGFB_MOUSE