Standard Curve (Sample)
(Mouse VEGFR2/KDR PicoKine ELISA Kit standard curve)
Mouse VEGFR2/KDR ELISA Kit | KDR elisa kit
Mouse VEGFR2/KDR PicoKine ELISA Kit
Gene Names
Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
Reactivity
Mouse
Synonyms
VEGFR2/KDR; N/A; Mouse VEGFR2/KDR PicoKine ELISA Kit; Vascular endothelial growth factor receptor 2; kinase insert domain receptor (a type III receptor tyrosine kinase); CD309; Fetal liver kinase 1; FLK1; KDR; Kinase insert domain receptor; Kinase insert domain receptor (a type III receptor tyrosine kinase); Protein tyrosine kinase receptor Flk 1; Tyrosine kinase growth factor receptor; VEGFR; VEGFR 2; VEGFR2; KDR elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse VEGFR2
Sequence Length
1367
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse VEGFR2/KDR PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse VEGFR2/KDR PicoKine ELISA Kit standard curve)
Related Product Information for KDR elisa kit
Principle of the assay: mouse VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGFR2 has been precoated onto 96-well plates. Standards(NSO, A20-E762) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGFR2 amount of sample captured in plate.
Background: Vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
Background: Vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
75,230 Da
NCBI Official Full Name
Vascular endothelial growth factor receptor 2
NCBI Official Synonym Full Names
kinase insert domain protein receptor
NCBI Official Symbol
Kdr
NCBI Official Synonym Symbols
Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
NCBI Protein Information
vascular endothelial growth factor receptor 2; kinase NYK; VEGF receptor-2; fetal liver kinase 1; protein-tyrosine kinase receptor flk-1; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; vascular endothelial growth factor receptor- 2; soluble vascular endothelial growth factor receptor 2
UniProt Protein Name
Vascular endothelial growth factor receptor 2
UniProt Gene Name
Kdr
UniProt Synonym Gene Names
Flk-1; Flk1; VEGFR-2; FLK-1
UniProt Entry Name
VGFR2_MOUSE
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Product Notes
The Mouse KDR kdr (Catalog #AAA45845) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45845 ELISA Kit recognizes Mouse KDR. It is sometimes possible for the material contained within the vial of "VEGFR2/KDR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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