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product-image-AAA108775_FCM11.jpg FCM/FACS (Flow Cytometry) (Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).)

Mouse anti-Human BLK Antibody | anti-BLK antibody

BLK Antibody

Gene Names
BLK; MODY11
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
BLK; N/A; BLK Antibody; MODY11; MGC10442; anti-BLK antibody
Ordering
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
1E6
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
505
Applicable Applications for anti-BLK antibody
ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant fragment of human BLK expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.

FCM/FACS (Flow Cytometry)

(Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).)

product-image-AAA108775_FCM11.jpg FCM/FACS (Flow Cytometry) (Figure 3: Flow cytometric analysis of HL-60 cells using BLK mouse mAb (green) and negative control (purple).)

IF (Immunofluorescence)

(Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

product-image-AAA108775_IF13.jpg IF (Immunofluorescence) (Figure 2: Immunofluorescence analysis of Hela cells using BLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

WB (Western Blot)

(Figure 1: Western blot analysis using BLK mAb against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.)

product-image-AAA108775_WB15.jpg WB (Western Blot) (Figure 1: Western blot analysis using BLK mAb against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.)
Related Product Information for anti-BLK antibody
Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development. Activation of B cells by various ligands is accompanied by activation of Blk. It has been suggested that Blk is involved in the control of B cell differentiation and proliferation. Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis. Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
640
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58kDa
NCBI Official Full Name
tyrosine-protein kinase Blk isoform 1
NCBI Official Synonym Full Names
BLK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
BLK
NCBI Official Synonym Symbols
MODY11
NCBI Protein Information
tyrosine-protein kinase Blk
UniProt Protein Name
Tyrosine-protein kinase Blk
UniProt Gene Name
BLK

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Product Notes

The BLK blk (Catalog #AAA108775) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BLK Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BLK can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the BLK blk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BLK, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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