Rabbit anti-Human, mouse BLK Polyclonal Antibody | anti-BLK antibody

BLK Antibody (N-term)

Cat. Num. AAA285523

• Gene Names: BLK; MODY11
• Reactivity: Human, mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: BLK, Antibody; BLK Antibody (N-term); Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk; BLK; anti-BLK antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This BLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BLK.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 505

• Applicable Applications for anti-BLK antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of anti-BLK Pab transiently transfected HEK-293 cell line lysate (1ug/lane). BLK pab(arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of anti-BLK Pab in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

WB (Western Blot)

(BLK Antibody (G1) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the BLK antibody detected the BLK protein (arrow).)

Related Product Information for anti-BLK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-BLK antibody

Cell Biology

References

Islam, K.B., et al., J. Immunol. 154(3):1265-1272 (1995).
Drebin, J.A., et al., Oncogene 10(3):477-486 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 33469982
• NCBI GeneID: 640
• NCBI Accession #: NP_001706.2
• NCBI GenBank Nucleotide #: NM_001715.2
• UniProt Accession #: P51451; Q16291; Q96IN1
• Molecular Weight: 57706
• NCBI Official Full Name: tyrosine-protein kinase Blk
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• UniProt Gene Name: BLK
• UniProt Entry Name: BLK_HUMAN