Mouse anti-Human CD172a Antibody | anti-CD172a antibody

Anti-Hu CD172a PerCP-Cy5.5

Cat. Num. AAA129369

• Gene Names: SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD172a; N/A; Anti-Hu CD172a PerCP-Cy5.5; signal regulatory protein alpha; PTPNS1, BIT, MFR, SIRPA, SHPS1; anti-CD172a antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG2a
• Clone Number: 15-414
• Specificity: The mouse monoclonal antibody 15-414 recognizes en extracellular epitope of CD172a (SIRP alpha), an approximately 90 kDa transmembrane glycoprotein expressed on cells of myeloid origin and neurons.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-CD172a antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Kg-1a cell line
• Conjugation: PerCP-Cy5.5
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD172a (15-414) PerCP-Cy5.5 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD172a (15-414) PerCP-Cy5.5 antibody (4ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD172a antibody

CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.

References

*Liu Y, Buhring HJ, Zen K, Burst SL, Schnell FJ, Williams IR, Parkos CA: Signal regulatory protein (SIRPalpha), a cellular ligand for CD47, regulates neutrophil transmigration. J Biol Chem. 2002 Mar 22;277(12):10028-36., URL: https://pubmed.ncbi.nlm.nih.gov/11792697/,*Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Buhring HJ: Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood. 2001 May 1;97(9):2741-9., URL: https://pubmed.ncbi.nlm.nih.gov/11313266/,
*Baba T, Badr Mel S, Tomaru U, Ishizu A, Mukaida N: Novel process of intrathymic tumor-immune tolerance through CCR2-mediated recruitment of Sirpalpha+ dendritic cells: a murine model. PLoS One. 2012;7(7):e41154. doi: 10.1371/journal.pone.0041154., URL: https://www.ncbi.nlm.nih.gov/pubmed/22815949,*Hansen IS, Krabbendam L, Bernink JH, Loayza-Puch F, Hoepel W, van Burgsteden JA, Kuijper EC, Buskens CJ, Bemelman WA, Zaat SAJ, Agami R, Vidarsson G, van den Brink GR, de Jong EC, Wildenberg ME, Baeten DLP, Everts B, den Dunnen J: FcalphaRI co-stimulation converts human intestinal CD103+ dendritic cells into pro-inflammatory cells through glycolytic reprogramming. Nat Commun. 2018 Feb 28;9(1):863., URL: https://pubmed.ncbi.nlm.nih.gov/29491406/,

NCBI and Uniprot Product Information

• NCBI GI #: 91105764
• NCBI GeneID: 140885
• NCBI Accession #: NP_001035111.1
• NCBI GenBank Nucleotide #: NM_001040022.1
• UniProt Accession #: P78324; O00683; O43799; Q8N517; Q8TAL8; Q9H0Z2; Q9UDX2; Q9UIJ6; Q9Y4U9; A2A2E1; A8K411; B2R6C3
• Molecular Weight: 54,967 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: SIRPA
• NCBI Official Synonym Symbols: BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-b
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Gene Name: SIRPA
• UniProt Synonym Gene Names: BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2
• UniProt Entry Name: SHPS1_HUMAN