Mouse anti-Human CD28 Antibody | anti-CD28 antibody

Anti-CD28 [15E8], Mouse IgG1

Cat. Num. AAA72296

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A affinity purified
• Synonyms: CD28; N/A; Anti-CD28 [15E8], Mouse IgG1; Anti-CD28 [15E8]; T-cell-specific surface glycoprotein CD28; TP44; anti-CD28 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1, Kappa
• Clone Number: 15E8
• Specificity: This antibody binds human CD28, which is found on a sub-population of T cells and activated B cells. The antibody does not react with B cells, granulocytes or monocytes. CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD28 antibody: FCM/FACS (Flow Cytometry), ELISA
• Immunogen: The original mouse antibody was generated by immunizing mice with human T lymphocytes.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

ELISA

(Binding curve of 15E8 (AAA72296) and YTH 913.12 to CD28. ELISA Plate coated with Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF (R&D systems) at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using AAA72296-23.0 or Ab00195-23.0. For detection, HRP-labelled goat anti-rabbit antibody was used.)

ELISA

(ELISA using 15E8 (AAA72296) and CD28. The plate was coated with CD28 at 2.5 ug/ml. Serial dilutions of AAA72296 were prepared over a range of 3000 ng/mL to 0.0169 ng/mL. For detection, HRP-labelled anti-mouse IgG antibody was used.)

Related Product Information for anti-CD28 antibody

This antibody works independently of TcR/CD3 and activation of protein kinase C (PKC) for activation of T cells and nehances cytokine secretion (PMID: 1647959). This antibody is capable of stimulating T cells in combination with either murine OKT3 antibody or CDR-mutated OKT3 Fab variants. It was found that cross-linking of specific tumor antigens with the T-cell-associated CD3 and CD28 antigens can increase IL-2 secretion, proliferation and antigen-specific cytotoxicity in resting T cells. This cross-linking can be achieved effectively by bispecific monoclonal antibodies (BiMAb) with specificity for both the tumor antigen and CD3 or CD28 antigen. A combination of bispecific antibodies BiMAb OKT3/HRS-3 with reactivity against both CD3 and Hodgkin's-lymphoma-associated CD30 antigen and BiMAb 15E8/HRS-3 with reactivity to both CD28 and CD30 antigen was able to activate resting T cells, represented by Jurkat cells (CD3+/CD28+) to produce IL-2 (PMID: 7686889). This antibody was also used in another study wherein the combined use of two CD3 x CD19 plus CD28 x CD22 bispecific antibodies induced optimal interleukin 2 secretion by Jurkat T-cell acute lymphocytic leukemia cells in the presence of target B-cells (PMID: 7689932). This antibody also provides a co-stimulatory signal to T cells. The scFv fragment of this antibody induced proliferation of peripheral blood mononuclear cells in the presence of anti-CD3 or anti-CD2 mAb and enhanced anti-tumor activity of anti-MUC1×anti-CD3 bispecific antibody when tested with lymphokine-activated killer cells with T cell phenotype (PMID: 10913626). The humanzied version of this antibody is available on request.

Product Categories/Family for anti-CD28 antibody

T cells; T-cell activation; T cell stimulation; bispecific antibody; lymphoma

References

Van Lier et al. T cell receptor/CD3 and CD28 use distinct intracellular signaling pathways/ Eur J Immunol. 1991 Jul;21(7):1775-8. PMID:1647959

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN