FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab (hu5c8, AAA72234) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Ruplizimab (AAA72234-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
Mouse anti-Human CD40L Antibody | anti-CD40L antibody
Anti-CD40L [hu5c8 (Ruplizumab)], Mouse IgG2a
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD40L; N/A; Anti-CD40L [hu5c8 (Ruplizumab)], Mouse IgG2a; Anti-CD40L [hu5c8 (Ruplizumab)]; CD154; CD40 ligand; tumour necrosis factor ligand superfamily member 5; TNFSF5; tumour necrosis factor related activation protein; TRAP; anti-CD40L antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2a, Kappa
Clone Number
hu5c8 (Ruplizumab)
Specificity
The antibody binds to CD40L with an IC50 of 0.845 ug/ml.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD40L antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), Blocking
Immunogen
Human CD40L
Origin Pub PMID
11525169
Chimeric Use Statement
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab (hu5c8, AAA72234) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Ruplizimab (AAA72234-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab (hu5c8, AAA72234) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Ruplizimab (AAA72234-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
WB (Western Blot)
(Western Blot using anti-OX40L antibody AAA72234 Rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with AAA72234-10.0 at 1 ug/ml before being detected by a secondary antibody. The expected band size (29 kDa) is seen in the human-derived samples, as well as bands corresponding to dimers and other multimers.)
WB (Western Blot)
(Western Blot using anti-OX40L antibody AAA72234 Rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with AAA72234-10.0 at 1 ug/ml before being detected by a secondary antibody. The expected band size (29 kDa) is seen in the human-derived samples, as well as bands corresponding to dimers and other multimers.)
Related Product Information for anti-CD40L antibody
The antibody binds specifically to CD40L, a surface receptor expressed on activated T cells which acts as a costimulatory molecule to trigger immune responses. The antibody neutralises CD40L function, as it blocks the interaction between CD40 and CD40L. When injected into cynomolgus monkeys, the original therapeutic antibody was found to have a T1/2 of 531? +/-?155 hours.
Product Categories/Family for anti-CD40L antibody
References
Karpusas et al. Structure of CD40 Ligand in Complex with the Fab Fragment of a Neutralizing Humanized Antibody Structure. 2001 Apr 4;9(4):321-9. PMID:11525169
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,274 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
NCBI Protein Information
CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Gene Name
CD40LG
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP
UniProt Entry Name
CD40L_HUMAN
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Product Notes
The CD40L cd40lg (Catalog #AAA72234) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40L [hu5c8 (Ruplizumab)], Mouse IgG2a reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD40L can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), Blocking. Researchers should empirically determine the suitability of the CD40L cd40lg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40L, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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