FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD98 antibody EP3-1 (AAA72246). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of EP3-1 (AAA72246-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Mouse anti-Human CD98 Antibody | anti-CD98 antibody
Anti-CD98 [EP3-1], Mouse IgG1
Gene Names
SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence
Purity
Protein A affinity purified
Synonyms
CD98; N/A; Anti-CD98 [EP3-1], Mouse IgG1; Anti-CD98 [EP3-1]; LAT1; E16; MPE16; SLC7A5; anti-CD98 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1, Kappa
Clone Number
EP3-1
Specificity
Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2, but showed no reactivity to live HeLa cells by IF.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD98 antibody
IP (Immunoprecipitation), IF (Immunofluorescence)
Immunogen
Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. CD98-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD98 antibody EP3-1 (AAA72246). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of EP3-1 (AAA72246-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD98 antibody EP3-1 (AAA72246). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of EP3-1 (AAA72246-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with antiCD98 (AAA72246) EP3-1 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (AAA72246-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72246-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with antiCD98 (AAA72246) EP3-1 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (AAA72246-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72246-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD98 antibody
CD98 may be a target for T or B cell immune responses against tumour cells in cancer patients (Itoh 2008).
Product Categories/Family for anti-CD98 antibody
References
Itoh Antibody epitope peptides as potential inducers of IgG antibodies against CD98 oncoprotein. Cancer Sci. 2009 Jan;100(1):126-31. PMID:19018757
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,010 Da
NCBI Official Full Name
large neutral amino acids transporter small subunit 1
NCBI Official Synonym Full Names
solute carrier family 7 (amino acid transporter light chain, L system), member 5
NCBI Official Symbol
SLC7A5
NCBI Official Synonym Symbols
E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
NCBI Protein Information
large neutral amino acids transporter small subunit 1; 4F2 LC; 4F2 light chain; CD98 light chain; integral membrane protein E16; L-type amino acid transporter 1; solute carrier family 7 member 5; large neutral amino acids transporter 1; y+ system cationic
UniProt Protein Name
Large neutral amino acids transporter small subunit 1
UniProt Gene Name
SLC7A5
UniProt Synonym Gene Names
CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1
UniProt Entry Name
LAT1_HUMAN
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Product Notes
The CD98 slc7a5 (Catalog #AAA72246) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD98 [EP3-1], Mouse IgG1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD98 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CD98 slc7a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD98, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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