ChIP (Chromatin Immunoprecipitation)
(AbFlex TWIST Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human brain tissue and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and sequence tags were mapped to identify TWIST binding sites across a region of chromosome 12.)
Mouse anti-Human TWIST Antibody | anti-TWIST antibody
AbFlex TWIST antibody (rAb)
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; SWCOS; TWIST; bHLHa38
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
TWIST; N/A; AbFlex TWIST antibody (rAb); CRS; SCS; ACS3; CRS1; BPES2; BPES3; TWIST1; bHLHa38; anti-TWIST antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-TWIST antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a recombinant protein corresponding to full length mouse TWIST1 (twist homolog 1 (Drosophila)).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
ChIP (Chromatin Immunoprecipitation)
(AbFlex TWIST Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human brain tissue and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and sequence tags were mapped to identify TWIST binding sites across a region of chromosome 12.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex TWIST Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human brain tissue and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and sequence tags were mapped to identify TWIST binding sites across a region of chromosome 12.)
SDS-PAGE
(AbFlex TWIST Antibody (rAb) tested by Western blot 50 ug of SH-SY5Y nuclear extract extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
SDS-PAGE
(AbFlex TWIST Antibody (rAb) tested by Western blot 50 ug of SH-SY5Y nuclear extract extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
Related Product Information for anti-TWIST antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex TWIST antibody (rAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex TWIST antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial-mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex TWIST antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial-mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.
Product Categories/Family for anti-TWIST antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22kDa
NCBI Official Full Name
twist-related protein 1
NCBI Official Synonym Full Names
twist family bHLH transcription factor 1
NCBI Official Symbol
TWIST1
NCBI Official Synonym Symbols
CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; SWCOS; TWIST; bHLHa38
NCBI Protein Information
twist-related protein 1
UniProt Protein Name
Twist-related protein 1
UniProt Gene Name
TWIST1
UniProt Synonym Gene Names
BHLHA38; TWIST; bHLHa38
UniProt Entry Name
TWST1_HUMAN
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Product Notes
The TWIST twist1 (Catalog #AAA60294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex TWIST antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TWIST can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the TWIST twist1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWIST, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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