WB (Western Blot)
(Detection of human TWIST1 by western blot. Samples: Whole cell lysate (50 ug) from SK-N-SH, HEK293T, SK-N-BE(2), HeLa, and SK-N-MC cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
TWIST Recombinant Antibody | TWIST1 recombinant antibody
Rabbit anti-TWIST Recombinant Monoclonal Antibody [BLR156J]
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
TWIST, Recombinant Antibody; Rabbit anti-TWIST Recombinant Monoclonal Antibody [BLR156J]; H-twist; twist-related protein 1; TWIST homolog of drosophila; twist homolog 1; twist basic helix-loop-helix transcription factor 1; TWIST; SWCOS; CSO; B-HLH DNA binding protein; CRS1; ACS3; CRS; class A basic helix-loop-helix protein 38; BPES3; BPES2; bHLHa38; SCS; Twist-related protein 1; twist family bHLH transcription factor 1; TWIST1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR156J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for TWIST1 recombinant antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TWIST1 by western blot. Samples: Whole cell lysate (50 ug) from SK-N-SH, HEK293T, SK-N-BE(2), HeLa, and SK-N-MC cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human TWIST1 by western blot. Samples: Whole cell lysate (50 ug) from SK-N-SH, HEK293T, SK-N-BE(2), HeLa, and SK-N-MC cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human TWIST1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-SH cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1) used for IP at 20 ul/mg lysate. TWIST1 was also immunoprecipitated by a second antibody against a different epitope of TWIST1 (Antibody 2). For blotting immunoprecipitated TWIST1, AAA213613 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TWIST1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-SH cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1) used for IP at 20 ul/mg lysate. TWIST1 was also immunoprecipitated by a second antibody against a different epitope of TWIST1 (Antibody 2). For blotting immunoprecipitated TWIST1, AAA213613 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
ICC (Immunocytochemistry)
(Detection of human TWIST1 in FFPE SK-N-BE(2) cells by immunocytochemistry. Antibody: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human TWIST1 in FFPE SK-N-BE(2) cells by immunocytochemistry. Antibody: Rabbit anti-TWIST1 recombinant monoclonal antibody (AAA213613 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for TWIST1 recombinant antibody
TWIST1 is a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. TWIST1 forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. TWIST1 may also regulate neural tube closure, limb development and brown fat metabolism. The TWIST1 gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. [taken from NCBI Entrez Gene (Gene ID: 7291)].
Product Categories/Family for TWIST1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,954 Da
NCBI Official Full Name
Homo sapiens twist family bHLH transcription factor 1 (TWIST1), mRNA
NCBI Official Synonym Full Names
twist family bHLH transcription factor 1
NCBI Official Symbol
TWIST1
NCBI Official Synonym Symbols
CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
NCBI Protein Information
twist-related protein 1; B-HLH DNA binding protein; H-twist; TWIST homolog of drosophila; class A basic helix-loop-helix protein 38; twist basic helix-loop-helix transcription factor 1; twist homolog 1
UniProt Protein Name
Twist-related protein 1
UniProt Gene Name
TWIST1
UniProt Synonym Gene Names
BHLHA38; TWIST; bHLHa38
UniProt Entry Name
TWST1_HUMAN
Similar Products
Product Notes
The TWIST1 twist1 (Catalog #AAA213613) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TWIST Recombinant Monoclonal Antibody [BLR156J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TWIST can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TWIST1 twist1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWIST, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.