DB (Dot Blot)
(AbFlex ATM phospho Ser1981 antibody specifity Dot blot analysis was used to confirm the specificity of ATM phospho Ser1981 antibody. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with antibody (0.5 ug/ml dilution). 1: ATM phospho serine 1981 (SLAFEEG(Sph)QSTTISS) 2: H2AX phospho serine 139 (CKATQA[Sph]QEY) 3: ATM unmodified serine 1981 (SLAFEEGSQSTTISS) 4: NBS phospho serine 343 (CPGPSL[Sph]QGVS) 5: BRCA1 phospho serine 1423 (LEQHG[Sph]QSNSC))
Mouse anti-Human, Mouse ATM phospho Ser1981 Antibody | anti-ATM antibody
AbFlex ATM phospho Ser1981 antibody (rAb)
Reactivity
Human, Mouse
Applications
Western Blot, Dot Blot
Purity
Protein A Chromatography
Synonyms
ATM phospho Ser1981; N/A; AbFlex ATM phospho Ser1981 antibody (rAb); ATM Serine/Threonine Kinase; Ataxia Telangiectasia Mutated 2 3 4 A-T Mutated; Ataxia Telangiectasia Mutated; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; anti-ATM antibody
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-ATM antibody
WB (Western Blot), DB (Dot Blot)
Antibody Type
Recombinant Antibody
Conjugation
Phosphorylated
Immunogen
This antibody was raised against A peptide surrounding phosph-serine 1981 of human ATM (Ataxia telangiectasia mutated).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(AbFlex ATM phospho Ser1981 antibody specifity Dot blot analysis was used to confirm the specificity of ATM phospho Ser1981 antibody. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with antibody (0.5 ug/ml dilution). 1: ATM phospho serine 1981 (SLAFEEG(Sph)QSTTISS) 2: H2AX phospho serine 139 (CKATQA[Sph]QEY) 3: ATM unmodified serine 1981 (SLAFEEGSQSTTISS) 4: NBS phospho serine 343 (CPGPSL[Sph]QGVS) 5: BRCA1 phospho serine 1423 (LEQHG[Sph]QSNSC))
DB (Dot Blot)
(AbFlex ATM phospho Ser1981 antibody specifity Dot blot analysis was used to confirm the specificity of ATM phospho Ser1981 antibody. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with antibody (0.5 ug/ml dilution). 1: ATM phospho serine 1981 (SLAFEEG(Sph)QSTTISS) 2: H2AX phospho serine 139 (CKATQA[Sph]QEY) 3: ATM unmodified serine 1981 (SLAFEEGSQSTTISS) 4: NBS phospho serine 343 (CPGPSL[Sph]QGVS) 5: BRCA1 phospho serine 1423 (LEQHG[Sph]QSNSC))
WB (Western Blot)
(AbFlex ATM phospho Ser1981 antibody (rAb) tested by Western blot. 20 ug HeLa nuclear extract (high salt preparation) untreated (lane 1) or treated with VP16 at 100 uM for 8 hrs (Lane 2), probed with 1 ug/ml ATM phospho Ser1981 antibody. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.)
WB (Western Blot)
(AbFlex ATM phospho Ser1981 antibody (rAb) tested by Western blot. 20 ug HeLa nuclear extract (high salt preparation) untreated (lane 1) or treated with VP16 at 100 uM for 8 hrs (Lane 2), probed with 1 ug/ml ATM phospho Ser1981 antibody. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.)
Related Product Information for anti-ATM antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex ATM phospho Ser1981 antibody (rAb) was raised in a Mouse host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human and Mouse samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. ATM-Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1, SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. ATM-Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1, SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.
Product Categories/Family for anti-ATM antibody
NCBI and Uniprot Product Information
Molecular Weight
370kDa
Similar Products
Product Notes
The ATM (Catalog #AAA60273) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex ATM phospho Ser1981 antibody (rAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATM phospho Ser1981 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot). Researchers should empirically determine the suitability of the ATM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM phospho Ser1981, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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