IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human ATM Polyclonal Antibody | anti-ATM antibody
ATM Antibody (N-term)
Gene Names
ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
ATM, Antibody; ATM Antibody (N-term); Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM; anti-ATM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This ATM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~34 amino acids from the N-terminal region of human ATM.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Length
3056
Applicable Applications for anti-ATM antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Cellular Location
Nucleus. Cytoplasmic vesicle Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
Tissue Location
Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes
Antigen Region
5-34
Preparation and Storage
Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Anti-ATM Antibody (C12) at 1:500 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 351 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ATM Antibody (C12) at 1:500 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 351 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ATM antibody
ATM is involved in signal transduction, cell cycle control and DNA repair, and may function as a tumor suppressor. It is necessary for activation of ABL1 and SAPK, and phosphorylates p53, NFKBIA, BRCA1, CTIP, NIBRIN (NBS1), TERF1, and RAD9. This protein has potential roles in vesicle and/or protein transport, T-cell development, gonad and neurological function. ATM is also part of the BRCA1-associated genome surveillance complex. ATM is induced by ionizing radiation. Defects in ATM are the cause of ataxia talangiectasia (AT), also known as Louis-Bar syndrome, a rare recessive disorder characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. About 30% of AT patients develop lymphomas and leukemias. Defects in ATM also contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. Defects in ATM also contribute to B-cell non-Hodgkin's lymphomas, and to B-cell chronic lymphocytic leukemia, a disease characterized by accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
Product Categories/Family for anti-ATM antibody
References
Suzuki, A., et al., J. Biol. Chem. 278 (1):48-53 (2003). Kishi, S., et al., J. Biol. Chem. 276 (31):29282-29291 (2001). Schaffner, C., et al., Proc. Natl. Acad. Sci. U.S.A. 97 (6):2773-2778 (2000). Gatei, M., et al., Nat. Genet. 25 (1):115-119 (2000). Becker-Catania, S.G., et al., Mol. Genet. Metab. 70 (2):122-133 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
serine-protein kinase ATM
NCBI Official Synonym Full Names
ATM serine/threonine kinase
NCBI Official Symbol
ATM
NCBI Official Synonym Symbols
AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
NCBI Protein Information
serine-protein kinase ATM
UniProt Protein Name
Serine-protein kinase ATM
UniProt Gene Name
ATM
UniProt Synonym Gene Names
A-T mutated
UniProt Entry Name
ATM_HUMAN
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Product Notes
The ATM atm (Catalog #AAA290715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATM Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATM can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATM atm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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