Mouse HLA-C Antibody | anti-HLA-C antibody
Anti-HLA-C Purified
Gene Names
HLA-C; HLC-C; D6S204; PSORS1; HLA-JY3
Reactivity
Non-human primates, Human
Applications
Immunoprecipitation, Flow Cytometry
Purity
Purified by protein-A affinity chromatography.
Synonyms
HLA-C; N/A; Anti-HLA-C Purified; HLC-C; D6S204; PSORS1; HLA-JY3; major histocompatibility complex; class I; C; anti-HLA-C antibody
Host
Mouse
Reactivity
Non-human primates, Human
Isotype
Mouse IgG2b
Clone Number
DT-9
Specificity
The mouse monoclonal antibody DT-9 recognizes an extracellular epitope on HLA-C member of MHC class I molecules. It does not crossreact with HLA-A or HLA-B allotypes.
Purity/Purification
Purified by protein-A affinity chromatography.
Form/Format
Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HLA-C antibody
Immunoprecipitation (IP), Flow Cytometry (FC)(QC tested)
Application Notes
FC: Recommended dilution: 1-4ug/ml.
Immunogen
Purified MHC class I molecules of tamarin origin
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
References
Korner C, Simoneau CR, Schommers P, Granoff M, Ziegler M, Hölzemer A, Lunemann S, Chukwukelu J, Corleis B, Naranbhai V, Kwon DS, Scully EP, Jost S, Kirchhoff F, Carrington M, Altfeld M: HIV-1-Mediated Downmodulation of HLA-C Impacts Target Cell Recognition and Antiviral Activity of NK Cells. Cell Host Microbe 2017 Jul 12;22(1):111-119., URL: https://pubmed.ncbi.nlm.nih.gov/28704647/
Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded beta2 m-free heavy chains. Eur J Immunol. 2015 Aug;45(8):2356-64., URL: https://pubmed.ncbi.nlm.nih.gov/25982269/
Serena M, Parolini F, Biswas P, Sironi F, Blanco Miranda A, Zoratti E, Scupoli MT, Ziglio S, Valenzuela-Fernandez A, Gibellini D, Romanellimg, Siccardi A, Malnati M, Beretta A, Zipeto D: HIV-1 Env associates with HLA-C free-chains at the cell membrane modulating viral infectivity. Sci Rep. 2018 May 22;8:46991. doi: 10.1038/srep46991., URL: https://pubmed.ncbi.nlm.nih.gov/29786690/
Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, McMichael A, Brackenridge S: Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011 Apr;85(7):3367-74., URL: https://pubmed.ncbi.nlm.nih.gov/21248048/
van der Ploeg K, Chang C, Ivarsson MA, Moffett A, Wills MR, Trowsdale J: Modulation of human leukocyte antigen-C by human cytomegalovirus stimulates KIR2DS1 recognition by natural killer cells. Front Immunol. 2017 Mar 29;8:298., URL: https://pubmed.ncbi.nlm.nih.gov/28424684/
Parolini F, Biswas P, Serena M, Sironi F, Muraro V, Guizzardi E, Cazzoletti L, Scupoli MT, Gibellini D, Ugolotti E, Biassoni R, Beretta A, Malnati M, Romanellimg, Zipeto D: Stability and expression levels of HLA-C on the cell membrane modulate HIV-1 infectivity. J Virol. 2017 Dec 14;92(1):e01711-17., URL: https://pubmed.ncbi.nlm.nih.gov/29070683/
Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded beta2 m-free heavy chains. Eur J Immunol. 2015 Aug;45(8):2356-64., URL: https://pubmed.ncbi.nlm.nih.gov/25982269/
Serena M, Parolini F, Biswas P, Sironi F, Blanco Miranda A, Zoratti E, Scupoli MT, Ziglio S, Valenzuela-Fernandez A, Gibellini D, Romanellimg, Siccardi A, Malnati M, Beretta A, Zipeto D: HIV-1 Env associates with HLA-C free-chains at the cell membrane modulating viral infectivity. Sci Rep. 2018 May 22;8:46991. doi: 10.1038/srep46991., URL: https://pubmed.ncbi.nlm.nih.gov/29786690/
Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, McMichael A, Brackenridge S: Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011 Apr;85(7):3367-74., URL: https://pubmed.ncbi.nlm.nih.gov/21248048/
van der Ploeg K, Chang C, Ivarsson MA, Moffett A, Wills MR, Trowsdale J: Modulation of human leukocyte antigen-C by human cytomegalovirus stimulates KIR2DS1 recognition by natural killer cells. Front Immunol. 2017 Mar 29;8:298., URL: https://pubmed.ncbi.nlm.nih.gov/28424684/
Parolini F, Biswas P, Serena M, Sironi F, Muraro V, Guizzardi E, Cazzoletti L, Scupoli MT, Gibellini D, Ugolotti E, Biassoni R, Beretta A, Malnati M, Romanellimg, Zipeto D: Stability and expression levels of HLA-C on the cell membrane modulate HIV-1 infectivity. J Virol. 2017 Dec 14;92(1):e01711-17., URL: https://pubmed.ncbi.nlm.nih.gov/29070683/
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
HLA class I histocompatibility antigen, Cw-1 alpha chain
NCBI Official Synonym Full Names
major histocompatibility complex, class I, C
NCBI Official Symbol
HLA-C
NCBI Official Synonym Symbols
HLC-C; D6S204; PSORS1; HLA-JY3
NCBI Protein Information
HLA class I histocompatibility antigen, Cw-1 alpha chain; HLA class I histocompatibility antigen, C alpha chain; MHC class I antigen heavy chain HLA-C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C
UniProt Protein Name
HLA class I histocompatibility antigen, Cw-7 alpha chain
UniProt Gene Name
HLA-C
UniProt Synonym Gene Names
HLAC
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Product Notes
The HLA-C hla-c (Catalog #AAA20035) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA-C Purified reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-C can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC)(QC tested). FC: Recommended dilution: 1-4ug/ml. Researchers should empirically determine the suitability of the HLA-C hla-c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-C, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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