FCM/FACS (Flow Cytometry)
(Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).)
Mouse anti-Human, Rat Pirh2 Antibody | anti-Pirh2 antibody
Pirh2 Antibody
Gene Names
RCHY1; ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
Reactivity
Human, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
Pirh2; N/A; Pirh2 Antibody; ARNIP; CHIMP; RNF199; RCHY1; anti-Pirh2 antibody
Host
Mouse
Reactivity
Human, Rat
Isotype
IgG1
Clone Number
1H10
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
212
Applicable Applications for anti-Pirh2 antibody
ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
FCM/FACS (Flow Cytometry)
(Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).)
FCM/FACS (Flow Cytometry)
(Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).)
IHC (Immunohiostchemistry)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb)
IHC (Immunohiostchemistry)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb)
WB (Western Blot)
(Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.)
WB (Western Blot)
(Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.)
Related Product Information for anti-Pirh2 antibody
Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N-and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Product Categories/Family for anti-Pirh2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30kDa; 60kDa
NCBI Official Synonym Full Names
ring finger and CHY zinc finger domain containing 1
NCBI Official Symbol
RCHY1
NCBI Official Synonym Symbols
ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
NCBI Protein Information
RING finger and CHY zinc finger domain-containing protein 1
UniProt Protein Name
RING finger and CHY zinc finger domain-containing protein 1
UniProt Gene Name
RCHY1
UniProt Synonym Gene Names
ARNIP; CHIMP; PIRH2; RNF199; ZNF363; hPirh2
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Product Notes
The Pirh2 rchy1 (Catalog #AAA108839) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Pirh2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pirh2 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Pirh2 rchy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pirh2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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