Mouse anti-Mammalian Neurofilament M Antibody | anti-NEFM antibody
Anti-Neurofilament M Purified
Gene Names
NEFM; NFM; NEF3; NF-M
Reactivity
Mammalian
Applications
Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
Neurofilament M; N/A; Anti-Neurofilament M Purified; neurofilament medium; NFM; NEFM; Neurofilament medium chain; NEF3; anti-NEFM antibody
Host
Mouse
Reactivity
Mammalian
Isotype
IgG2a
Clone Number
NF-09
Specificity
The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa intracellular antigen) of various species.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
916
Applicable Applications for anti-NEFM antibody
ICC (Immunocytochemistry), WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-NEFM antibody
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights - neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neurofilament medium polypeptide isoform 1
NCBI Official Synonym Full Names
neurofilament medium
NCBI Official Symbol
NEFM
NCBI Official Synonym Symbols
NFM; NEF3; NF-M
NCBI Protein Information
neurofilament medium polypeptide
UniProt Protein Name
Neurofilament medium polypeptide
UniProt Gene Name
NEFM
UniProt Synonym Gene Names
NEF3; NFM; NF-M
UniProt Entry Name
NFM_HUMAN
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Product Notes
The NEFM nefm (Catalog #AAA128900) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Neurofilament M Purified reacts with Mammalian and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament M can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NEFM nefm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament M, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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