IgG1 negative control

Mouse IgG1 Negative Control: Amethyst Orange

Cat. Num. AAA12275

• Applications: Flow Cytometry, Functional Assay
• Purity: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: IgG1; N/A; Mouse IgG1 Negative Control: Amethyst Orange; Mouse IgG1 Negative Control antibody; IgG1 negative control

• Host: Mouse
• Isotype: IgG1
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG conjugated to Amethyst Orange - Liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)

• Applicable Applications for IgG1 negative control: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; This product should be stored undiluted.; Storage in frost free freezers is not recommended.; This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody.; This product contains a precipitate we recommend microcentrifugation before use.

Application Data

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Application Data

(Figure A. A488 conjugated Mouse anti Human CD38 and RPE-A647 tandem conjugated Mouse IgG1 isotype control . Figure B. A488 conjugated Mouse anti Human CD38 and RPE-Cy5 tandem conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)

Application Data

(Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse IgG1 isotype control . Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 and A488 conjugated Mouse anti Human CD38 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)

Related Product Information for IgG1 negative control

Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012), lapine (Pakandl et al, 2008) and guinea-pig tissues.

Product Categories/Family for IgG1 negative control

Negative/Isotype Control