IF (Immunofluorescence)
(Immunofluorescence staining of mouse splenocytes with anti-XCR1 antibody MARX10 . Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MARX10 (AAA72351) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72351, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Mouse, Rat XCR1 Antibody | anti-XCR1 antibody
Anti-XCR1 [MARX10]
Gene Names
Xcr1; Gpr5; mXcr1; Ccxcr1
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified. Protein A affinity purified
Synonyms
XCR1; N/A; Anti-XCR1 [MARX10]; mXCR1; Ccxcr1; Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; MARX1O; anti-XCR1 antibody
Reactivity
Mouse, Rat
Isotype
Rabbit IgG kappa
Clone Number
MARX10
Specificity
This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-XCR1 antibody
Invitro Assay, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 x 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat- inactivated B. pertussis.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of mouse splenocytes with anti-XCR1 antibody MARX10 . Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MARX10 (AAA72351) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72351, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse splenocytes with anti-XCR1 antibody MARX10 . Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MARX10 (AAA72351) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72351, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-XCR1 antibody
The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 × 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.
XCR1 expression determines Batf3-dependent lineage of dendritic cells with a special capacity to cross-present antigen. The paper studies the expression of XCR1 on CD8+ dendritic cells using antibody MARX10 and its role in antigen presentation.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
XCR1 expression determines Batf3-dependent lineage of dendritic cells with a special capacity to cross-present antigen. The paper studies the expression of XCR1 on CD8+ dendritic cells using antibody MARX10 and its role in antigen presentation.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-XCR1 antibody
References
Bachem et al.
Front Immunol. 2012 Jul 18;3:214.
Front Immunol. 2012 Jul 18;3:214.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Chemokine XC receptor 1
NCBI Official Synonym Full Names
chemokine (C motif) receptor 1
NCBI Official Symbol
Xcr1
NCBI Official Synonym Symbols
Gpr5; mXcr1; Ccxcr1
NCBI Protein Information
chemokine XC receptor 1; SCM1 receptor; lymphotactin receptor; XC chemokine receptor 1; C motif-1/lymphotactin receptor; chemokine (C motif) XC receptor 1
UniProt Protein Name
Chemokine XC receptor 1
UniProt Gene Name
Xcr1
UniProt Synonym Gene Names
Ccxcr1
UniProt Entry Name
XCR1_MOUSE
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Product Notes
The XCR1 xcr1 (Catalog #AAA72351) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-XCR1 [MARX10] reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XCR1 can be used in a range of immunoassay formats including, but not limited to, Invitro Assay, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the XCR1 xcr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XCR1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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