Mouse Endothelin 1 (EDN1) RTU ELISA Kit | EDN1 rtu elisa kit
Mouse Endothelin 1 (EDN1) ELISA Kit
Gene Names
Edn1; ET-1; PPET1; preproET
Reactivity
Mouse
Synonyms
Endothelin 1 (EDN1); N/A; Mouse Endothelin 1 (EDN1) ELISA Kit; ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1 rtu elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of E3. No significant cross-reactivity or interference between E3 and analogues was observed.
Sequence Length
202
Samples
General Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
Assay Type
Quantitative Competitive
Detection Range
1.56-100ng/mL
Sensitivity
0.52ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Related Product Information for EDN1 rtu elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of E3 in general serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to E3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled E3 and unlabeled E3 (Standards or samples) with the pre-coated antibody specific to E3. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of E3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of E3 in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to E3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled E3 and unlabeled E3 (Standards or samples) with the pre-coated antibody specific to E3. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of E3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of E3 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,809 Da
NCBI Official Full Name
endothelin-1 preproprotein
NCBI Official Synonym Full Names
endothelin 1
NCBI Official Symbol
Edn1
NCBI Official Synonym Symbols
ET-1; PPET1; preproET
NCBI Protein Information
endothelin-1
UniProt Protein Name
Endothelin-1
UniProt Gene Name
Edn1
UniProt Synonym Gene Names
ET-1; PPET1
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Product Notes
The Mouse EDN1 edn1 (Catalog #AAA70349) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA70349 RTU ELISA Kit recognizes Mouse EDN1. It is sometimes possible for the material contained within the vial of "Endothelin 1 (EDN1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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