Standard Curve (Sample)
(Human CD206 PicoKine ELISA Kit standard curve)
Human CD206/CLEC13D ELISA Kit | MRC1 elisa kit
Human CD206/CLEC13D PicoKine Quick ELISA Kit
Gene Names
MRC1; MMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
Reactivity
Human
Applications
ELISA
Synonyms
CD206/CLEC13D; N/A; Human CD206/CLEC13D PicoKine Quick ELISA Kit; MRC1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human MRC1
Assay Type
Sandwich
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<50 pg/ml
Applicable Applications for MRC1 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human CD206 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CD206 PicoKine ELISA Kit standard curve)
Related Product Information for MRC1 elisa kit
Background: The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). This gene is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.
Principle of the Assay: The Quick Picokine™ Human MRC1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MRC1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human MRC1 protein as standards. To measure Human MRC1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human MRC1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MRC1 in the sample.
Principle of the Assay: The Quick Picokine™ Human MRC1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MRC1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human MRC1 protein as standards. To measure Human MRC1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human MRC1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MRC1 in the sample.
Product Categories/Family for MRC1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
166,012 Da
NCBI Official Full Name
macrophage mannose receptor 1
NCBI Official Synonym Full Names
mannose receptor, C type 1
NCBI Official Symbol
MRC1
NCBI Official Synonym Symbols
MMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
NCBI Protein Information
macrophage mannose receptor 1; mannose receptor, C type 1-like 1; C-type lectin domain family 13 member D; macrophage mannose receptor 1-like protein 1
UniProt Protein Name
Macrophage mannose receptor 1
UniProt Gene Name
MRC1
UniProt Synonym Gene Names
CLEC13D; CLEC13DL; MRC1L1; MMR
UniProt Entry Name
MRC1_HUMAN
Similar Products
Product Notes
The Human MRC1 mrc1 (Catalog #AAA126851) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126851 ELISA Kit recognizes Human MRC1. AAA Biotech's CD206/CLEC13D can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the MRC1 mrc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD206/CLEC13D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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