Mucin 1 (MUC1) Recombinant Protein | MUC1 recombinant protein
Recombinant Mucin 1 (MUC1)
Gene Names
Muc1; EMA; CD227; Muc-1
Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Mucin 1 (MUC1); N/A; Recombinant Mucin 1 (MUC1); MUC1 recombinant protein
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 97%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose
Concentration
Original Concentration: 300ug/mL (varies by lot)
Applicable Applications for MUC1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Ser410~Ala525
Tags
N-terminal His Tag
Subcellular Location
Membrane, Nucleus, Chromosome
Traits
Freeze-dried powder
Predicted isoelectric point
6.0
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
Sequence Information
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 14.6kDa
Accurate Molecular Mass: 10kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 10kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
mucin-1
NCBI Official Synonym Full Names
mucin 1, transmembrane
NCBI Official Symbol
Muc1
NCBI Official Synonym Symbols
EMA; CD227; Muc-1
NCBI Protein Information
mucin-1; episialin
UniProt Protein Name
Mucin-1
UniProt Gene Name
Muc1
UniProt Synonym Gene Names
Muc-1; MUC-1; MUC1-NT; MUC1-alpha; MUC1-beta
UniProt Entry Name
MUC1_MOUSE
