Resistin active protein
Human Resistin Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Resistin; N/A; Human Resistin Recombinant; RETN; FIZZ3; HXCP1; RSTN; Resistin active protein
Host
N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant human Resistin was lyophilized from a 0.2 um filtered PBS solution.
Sequence Length
108
Host Note
Optimized DNA sequence encoding Human Resistin mature chain was expressed in Escherichia Coli.
Biological Activity
Determined by its ability to stimulate lipolysis in cultured human adipocytes.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human Resistin, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 10 kDa.
Recombinant Resistin is a disulfide-linked homodimeric protein consisting of two 93 amino acid residue subunits, and migrates as an approximately 20 kDa protein under non-reducing conditions and as a 10 kDa protein under reducing conditions in SDS-PAGE.
and propeptide, the molecule has a calculated molecular mass of approximately 10 kDa.
Recombinant Resistin is a disulfide-linked homodimeric protein consisting of two 93 amino acid residue subunits, and migrates as an approximately 20 kDa protein under non-reducing conditions and as a 10 kDa protein under reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2°-8° for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2°-8° for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for Resistin active protein
Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down- regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target- tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90 % in 3T3-L1 adipocytes.
Product Categories/Family for Resistin active protein
References
• Resistin regulates pituitary somatotrope cell function through the activation of multiple signaling pathways .Endocrinology, Oct 2009; 150: 4643 - 4652.
• Resistin: a newly identified chemokine for human cd4-positive lymphocytes .Cardiovasc Res, Sep 2009; 10.1093/cvr/cvp278.
• Resistin-like molecule-β inhibits sglt-1 activity and enhances glut2-dependent jejunal glucose transport .Diabetes, Sep 2009; 58: 2032 - 2038.
• Resistin is more abundant in liver than adipose tissue and is not up-regulated by lipopolysaccharide .J. Clin. Endocrinol. Metab., Aug 2009; 94: 3051 - 3057.
• endoplasmic reticulum stress regulates adipocyte Resistin expression .Diabetes, Aug 2009; 58: 1879 - 1886.
• Resistin: a newly identified chemokine for human cd4-positive lymphocytes .Cardiovasc Res, Sep 2009; 10.1093/cvr/cvp278.
• Resistin-like molecule-β inhibits sglt-1 activity and enhances glut2-dependent jejunal glucose transport .Diabetes, Sep 2009; 58: 2032 - 2038.
• Resistin is more abundant in liver than adipose tissue and is not up-regulated by lipopolysaccharide .J. Clin. Endocrinol. Metab., Aug 2009; 94: 3051 - 3057.
• endoplasmic reticulum stress regulates adipocyte Resistin expression .Diabetes, Aug 2009; 58: 1879 - 1886.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
resistin
UniProt Protein Name
Resistin
UniProt Gene Name
RETN
UniProt Synonym Gene Names
FIZZ3; HXCP1; RSTN; ADSF
UniProt Entry Name
RETN_HUMAN
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Product Notes
The Resistin retn (Catalog #AAA14423) is an Active Protein produced from N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Resistin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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