Standard Curve (Sample)
Human Visfatin ELISA Kit | NAMPT elisa kit
Human Visfatin ELISA Kit
Gene Names
NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
Reactivity
Human
Applications
ELISA
Synonyms
Visfatin; N/A; Human Visfatin ELISA Kit; PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; NAMPT elisa kit
Reactivity
Human
Specificity
The Human Visfatin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Visfatin proteins within the range of 1560 pg/ml - 100000 pg/ml.
Sequence Length
491
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
700 pg/ml.
Applicable Applications for NAMPT elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NAMPT elisa kit
Principle of the Assay||The Human Visfatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Visfatin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Visfatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Visfatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Visfatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Visfatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Pre-B cell-enhancing factor (PBEF), also designated nicotinamide phosphoribosyltransferase (Nampt) or visfatin, belongs to the NAPRTase family of proteins. PBEF may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. It is involved in the catalysis of nicotinamide with 5-phosphoribosyl-1-pyrophosphate, yielding nicotinamide mononucleotide, which is important in NAD biosynthesis. This is a rate limiting step in the NAD biosynthesis pathway. Highly enriched in the visceral fat of both human and mice, PBEF expression levels in plasma increase during the development of obesity. PBEF is a cytoplasmic protein expressed primarily in bone marrow, muscle and liver tissue, but it can also be detected in placenta, lung, kidney and heart tissue.
Background/Introduction: Pre-B cell-enhancing factor (PBEF), also designated nicotinamide phosphoribosyltransferase (Nampt) or visfatin, belongs to the NAPRTase family of proteins. PBEF may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. It is involved in the catalysis of nicotinamide with 5-phosphoribosyl-1-pyrophosphate, yielding nicotinamide mononucleotide, which is important in NAD biosynthesis. This is a rate limiting step in the NAD biosynthesis pathway. Highly enriched in the visceral fat of both human and mice, PBEF expression levels in plasma increase during the development of obesity. PBEF is a cytoplasmic protein expressed primarily in bone marrow, muscle and liver tissue, but it can also be detected in placenta, lung, kidney and heart tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,521 Da
NCBI Official Full Name
Nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
NCBI Protein Information
nicotinamide phosphoribosyltransferase
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
UniProt Entry Name
NAMPT_HUMAN
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Product Notes
The Human NAMPT nampt (Catalog #AAA261775) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261775 ELISA Kit recognizes Human NAMPT. AAA Biotech's Visfatin can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the NAMPT nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Visfatin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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