Hamster Neutrophil Elastase ELISA Kit | NE elisa kit

Hamster Neutrophil Elastase ELISA Kit

Cat. Num. AAA34345

• Gene Names: ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• Reactivity: Hamster
• Synonyms: Neutrophil Elastase; N/A; Hamster Neutrophil Elastase ELISA Kit; NE elisa kit

• Reactivity: Hamster
• Specificity: No significant cross-reactivity or interference between Hamster NE and analogues was observed.
• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 25 ng/ml - 800 ng/ml.
• Sensitivity: 5.0 ng/ml.

• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NE concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster NE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NE and NE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NE elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NE concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster NE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NE and NE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503549
• NCBI GeneID: 1991
• NCBI Accession #: NP_001963.1
• NCBI GenBank Nucleotide #: NM_001972.2
• UniProt Accession #: P08246; P09649; Q6B0D9; Q6LDP5
• Molecular Weight: 28,518 Da
• NCBI Official Full Name: neutrophil elastase preproprotein
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: ELANE
• NCBI Official Synonym Symbols: GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• NCBI Protein Information: neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
• UniProt Protein Name: Neutrophil elastase
• UniProt Gene Name: ELANE
• UniProt Synonym Gene Names: ELA2; HLE
• UniProt Entry Name: ELNE_HUMAN