Application Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
NFkB1 p50 Recombinant Protein | NFkB1 recombinant protein
Recombinant NFkB1 p50 (1-434) protein
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Synonyms
NFkB1 p50; N/A; Recombinant NFkB1 p50 (1-434) protein; CVID12; EBP-1; NF-kB1; NF-kappa-B; NFkB1 recombinant protein
Host
E Coli
Form/Format
Recombinant NFKB1/p50 (1-434) protein is supplied at a concentration of 0.9ug/ul in 25mM Tris pH8.0, 300mM NaCl, 10% glycerol, 0.5mM TCEP.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant NFKB1/p50 (1-434) protein is suitable for use in protein-protein interaction, in vitro transcription assay, binding assay.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Application Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Application Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
Application Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
SDS-PAGE
(Recombinant NFKB1 / p50 (1-434) protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 73.9 kDa Purity: ?8 )
SDS-PAGE
(Recombinant NFKB1 / p50 (1-434) protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 73.9 kDa Purity: ?8 )
Related Product Information for NFkB1 recombinant protein
Short Description: Recombinant NFkB1 p50 (1-434) protein including amino acid 1-434 of NFkB1 p105 subunit (accession number NP_003989.2) was expressed in E Coli with an N-terminal GST-Tag. The molecular weight of NFkB1 p50 (1-434) is 73.9 kDa. It is suitable for use in protein-protein interaction studies, in vitro transcription assays and binding assays.
Background: NFKB1 (Nuclear Factor Kappa B Subunit 1, also known as CVID12, EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkB, p105, p50) is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. The precursor NFKB1 is a 105 kDa protein (p105). The 105 kDa protein can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein (p50). The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB complex plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.
Background: NFKB1 (Nuclear Factor Kappa B Subunit 1, also known as CVID12, EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkB, p105, p50) is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. The precursor NFKB1 is a 105 kDa protein (p105). The 105 kDa protein can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein (p50). The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB complex plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.
Product Categories/Family for NFkB1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,520 Da
NCBI Official Full Name
nuclear factor NF-kappa-B p105 subunit isoform 1
NCBI Official Synonym Full Names
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
NCBI Official Symbol
NFKB1
NCBI Official Synonym Symbols
p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
NCBI Protein Information
nuclear factor NF-kappa-B p105 subunit; NF-kappabeta; DNA binding factor KBF1; DNA-binding factor KBF1; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit
UniProt Protein Name
Nuclear factor NF-kappa-B p105 subunit
UniProt Gene Name
NFKB1
UniProt Entry Name
NFKB1_HUMAN
Similar Products
Product Notes
The NFkB1 nfkb1 (Catalog #AAA60112) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NFkB1 p50, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.