NGF recombinant protein

NGF Protein, Human, Recombinant

Cat. Num. AAA257435

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: NGF; N/A; NGF Protein, Human, Recombinant; Human beta-NGF/Beta-NGF Protein; nerve growth factor (beta polypeptide); Beta-NGF Protein, Human; HSAN5 Protein, Human; NGFB Protein, Human; NGF recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 40mM His, 40mM Arg, 150mM NaCl, pH5.5 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 118aa
• Sequence: Ser122-Arg239

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser122
• Protein Construction: |A DNA sequence encoding the mature form of human beta-NGF (NP_002497.2) (Ser 122-Arg 239) was expressed
• Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2ng/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.)

Application Data

Related Product Information for NGF recombinant protein

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, alpha, beta, and gamma, among which, the beta subunit, called beta-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Product Categories/Family for NGF recombinant protein

Death Receptor Signaling

References

Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.

NCBI and Uniprot Product Information

• NCBI GI #: 70995319
• NCBI GeneID: 4803
• NCBI Accession #: NP_002497.2
• NCBI GenBank Nucleotide #: NM_002506.2
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF
• NCBI Protein Information: beta-nerve growth factor; nerve growth factor, beta subunit
• UniProt Protein Name: Beta-nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB; Beta-NGF
• UniProt Entry Name: NGF_HUMAN