Standard Curve (Sample)
Human Nidogen 1 ELISA Kit | NID1 elisa kit
Human Nidogen 1 ELISA Kit
Gene Names
NID1; NID
Reactivity
Human
Applications
ELISA
Synonyms
Nidogen 1; N/A; Human Nidogen 1 ELISA Kit; NID; Nidogen-1; NID-1; Entactin; NID1 elisa kit
Reactivity
Human
Specificity
The Human Nidogen 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Nidogen 1 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
1247
Samples
Culture Supernatants, Serum
Sensitivity
< 10 pg/ml
Applicable Applications for NID1 elisa kit
Sandwich ELISA (SE)
Detection Method
Colorimetric
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NID1 elisa kit
Background: Nidogen-1 (NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.
Principle of the Assay: The Human Nidogen 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Nidogen 1 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Nidogen 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Nidogen 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Nidogen 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Nidogen 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human Nidogen 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Nidogen 1 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Nidogen 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Nidogen 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Nidogen 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Nidogen 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,017 Da
NCBI Official Full Name
nidogen-1
NCBI Official Synonym Full Names
nidogen 1
NCBI Official Symbol
NID1
NCBI Official Synonym Symbols
NID
NCBI Protein Information
nidogen-1; NID-1; enactin; entactin
UniProt Protein Name
Nidogen-1
UniProt Gene Name
NID1
UniProt Synonym Gene Names
NID; NID-1
UniProt Entry Name
NID1_HUMAN
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Product Notes
The Human NID1 nid1 (Catalog #AAA17881) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17881 ELISA Kit recognizes Human NID1. AAA Biotech's Nidogen 1 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the NID1 nid1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nidogen 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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