Nidogen-1 Recombinant Protein | Nid1 recombinant protein

Recombinant Mouse Nidogen-1

Cat. Num. AAA113461

• Gene Names: Nid1; Nid; entactin; nidogen-1; A630025O17; entactin-1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Nidogen-1; N/A; Recombinant Mouse Nidogen-1; Entactin; Nid1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 428-665aa; Partial
• Sequence: SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA
• Sequence Length: 665

• Production Note: Special Offer: The Yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeasthost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Yeast host-expressed protein for the fastest delivery among all hosts. Please contact our or email to for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for Nid1 recombinant protein

Sulfated glycoprotein widely distributed in basent membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

References

Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor.Durkin M.E., Chakravarti S., Bartos B.B., Liu S.H., Friedman R.L., Chung A.E.J. Cell Biol. 107:2749-2756(1988) Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells.Mann K., Deutzmann R., Aumailley M., Timpl R., Raimondi L., Yamamda Y., Pan T.-C., Conway D., Chu M.-L.EMBO J. 8:65-72(1989) Characterization of the 5' end of the mouse Ent gene encoding the basement membrane protein, entactin.Durkin M.E., Liu S.H., Reing J., Chung A.E.Gene 132:261-266(1993) Exon organization of the mouse entactin gene corresponds to the structural domains of the polypeptide and has regional homology to the low-density lipoprotein receptor gene.Durkin M.E., Wewer U.M., Chung A.E.Genomics 26:219-228(1995) Purification and structural characterization of intact and fragmented nidogen obtained from a tumor basement membrane.Paulsson M., Deutzmann R., Dziadek M., Nowack H., Timpl R., Weber S., Engel J.Eur. J. Biochem. 156:467-478(1986) Structure and localization of O- and N-linked oligosaccharide chains on basement membrane protein nidogen.Fujiwara S., Shinkai H., Mann K., Timpl R.Matrix 13:215-222(1993) Binding of fibulin-1 to nidogen depends on its C-terminal globular domain and a specific array of calcium-binding epidermal growth factor-like (EG) modules.Adam S., Goehring W., Wiedemann H., Chu M.-L., Timpl R., Kostka G.J. Mol. Biol. 272:226-236(1997) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.Sasaki T., Brakebusch C., Engel J., Timpl R.EMBO J. 17:1606-1613(1998) Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies.Ries A., Goehring W., Fox J.W., Timpl R., Sasaki T.Eur. J. Biochem. 268:5119-5128(2001) Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo.Lee H.K., Seo I.A., Park H.K., Park H.T.FEBS Lett. 580:2253-2257(2006) Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1.Hopf M., Gohring W., Ries A., Timpl R., Hohenester E.Nat. Struct. Biol. 8:634-640(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 171543883
• NCBI GeneID: 18073
• NCBI Accession #: NP_035047.2
• NCBI GenBank Nucleotide #: NM_010917.2
• UniProt Accession #: P10493; Q3TKX9; Q8BQI3; Q8C3U8; Q8C9P6
• Molecular Weight: 28.3 kDa
• NCBI Official Full Name: nidogen-1
• NCBI Official Synonym Full Names: nidogen 1
• NCBI Official Symbol: Nid1
• NCBI Official Synonym Symbols: Nid; entactin; nidogen-1; A630025O17; entactin-1
• NCBI Protein Information: nidogen-1
• UniProt Protein Name: Nidogen-1
• UniProt Gene Name: Nid1
• UniProt Synonym Gene Names: Ent; NID-1
• UniProt Entry Name: NID1_MOUSE