Ribonucleoside-diphosphate reductase 1 subunit alpha Recombinant Protein | nrdA recombinant protein

Recombinant Escherichia coli Ribonucleoside-diphosphate reductase 1 subunit alpha

Cat. Num. AAA116119

• Gene Names: nrdA; dnaF; ECK2226; JW2228
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ribonucleoside-diphosphate reductase 1 subunit alpha; N/A; Recombinant Escherichia coli Ribonucleoside-diphosphate reductase 1 subunit alpha; Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1; nrdA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-761aa; Full Length
• Sequence: MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELRSHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPALYDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQRVKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACPWFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAEDAQDDLVPSIQDDGCESGACKI
• Sequence Length: 761

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for nrdA recombinant protein

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide. It also provides redox-active cysteines.

References

Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.Carlson J., Fuchs J.A., Messing J.Proc. Natl. Acad. Sci. U.S.A. 81:4294-4297(1984) Nucleotide sequence of the gene coding for the large subunit of ribonucleotide reductase of Escherichia coli. Correction.Nilsson O., Aaberg A., Lundqvist T., Sjoeberg B.-M.Nucleic Acids Res. 16:4174-4174(1988) Sjoeberg B.-M.Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.DNA Res. 4:91-113(1997) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Protein B1 of ribonucleotide reductase. Direct analytical data and comparisons with data indirectly deduced from the nucleotide sequence of the Escherichia coli nrdA gene.Sjoeberg B.-M., Eriksson S., Joernvall H., Carlquist M., Eklund H.Eur. J. Biochem. 150:423-427(1985) Evidence for two different classes of redox-active cysteines in ribonucleotide reductase of Escherichia coli.Aaberg A., Hahne S., Karlsson M., Larsson A., Ormoe M., Aahgren A., Sjoeberg B.-M.J. Biol. Chem. 264:12249-12252(1989) Two conserved tyrosine residues in protein R1 participate in an intermolecular electron transfer in ribonucleotide reductase.Ekberg M., Sahlin M., Eriksson M., Sjoberg B.M.J. Biol. Chem. 271:20655-20659(1996) Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.Mol. Cell. Proteomics 8:215-225(2009) Structure of ribonucleotide reductase protein R1.Uhlin U., Eklund H.Nature 370:533-539(1994) A new mechanism-based radical intermediate in a mutant R1 protein affecting the catalytically essential Glu441 in Escherichia coli ribonucleotide reductase.Persson A.L., Eriksson M., Katterle B., Potsch S., Sahlin M., Sjoberg B.M.J. Biol. Chem. 272:31533-31541(1997) Binding of allosteric effectors to ribonucleotide reductase protein R1 reduction of active-site cysteines promotes substrate binding.Eriksson M., Uhlin U., Ramaswamy S., Ekberg M., Regnstroem K., Sjoeberg B.-M., Eklund H.Structure 5:1077-1092(1997) Binding specificity and mechanistic insight into glutaredoxin-catalyzed protein disulfide reduction.Berardi M.J., Bushweller J.H.J. Mol. Biol. 292:151-161(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 16130169
• NCBI GeneID: 946612
• NCBI Accession #: NP_416737.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P00452; P78088; P78177
• Molecular Weight: 101.8 kDa
• NCBI Official Full Name: ribonucleoside-diphosphate reductase 1, alpha subunit
• NCBI Official Symbol: nrdA
• NCBI Official Synonym Symbols: dnaF; ECK2226; JW2228
• NCBI Protein Information: ribonucleoside-diphosphate reductase 1, alpha subunit
• UniProt Protein Name: Ribonucleoside-diphosphate reductase 1 subunit alpha
• UniProt Gene Name: nrdA
• UniProt Synonym Gene Names: dnaF
• UniProt Entry Name: RIR1_ECOLI