Rat neurotrophin 3 ELISA Kit | NTF3 elisa kit

Rat Neurotrophin 3, NT-3 ELISA Kit

Cat. Num. AAA80060

• Reactivity: Rat
• Synonyms: neurotrophin 3; N/A; Rat Neurotrophin 3, NT-3 ELISA Kit; Rat Neurotrophin 3; NT-3 ELISA KIT; HDNF; MGC129711; NGF-2; NGF2; NT3; ; NTF3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat NTF3. No significant cross-reactivity or interference between Rat NTF3 and analogues was observed.
• Sequence Length: 271
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.9ng/ml-60ng/ml
• Sensitivity: 0.225 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for NTF3 elisa kit

Introduction: Neurotrophin-3, or NT-3 is a neurotrophic factor, in the NGF (Nerve Growth Factor)-family of neurotrophins. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. NT-3 was the third neurotrophic factor to be characterized, after nerve growth factor (NGF) and BDNF (Brain Derived Neurotrohic Factor). Although the vast majority of neurons in the mammalian brain are formed prenatally, parts of the adult brain retain the ability to grow new neurons from neural stem cells; a process known as neurogenesis. Neurotrophins are chemicals that help to stimulate and control neurogenesis. NT-3 is unique in the number of neurons it can potentially stimulate, given its ability to activate two of the receptor tyrosine kinase neurotrophin receptors (TrkC and TrkB - see below). Mice born without the ability to make NT-3 have loss of proprioceptive and subsets of mechanoreceptive sensory neurons. NT-3 binds three receptors on the surface of cells which are capable of responding to this growth factor:TrkC (pronounced "Track C"), is apparently the "physiologic" receptor, in that it binds with greatest affinity to NT-3. However, NT-3 is capable of binding and signaling through a TrkC-related receptors called TrkB. Finally, NT-3 also binds a second-receptor type besides Trk receptors, called the LNGFR (for "low affinity nerve growth factor receptor).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NT-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NT-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NT-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NT-3 in the samples is then determined by 4 comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 399498552
• NCBI GeneID: 81737
• NCBI Accession #: NP_001257797.1
• NCBI GenBank Nucleotide #: NM_001270868.1
• UniProt Accession #: P18280; Q6NS33
• Molecular Weight: 29,645 Da
• NCBI Official Full Name: neurotrophin-3 isoform 2
• NCBI Official Synonym Full Names: neurotrophin 3
• NCBI Official Symbol: Ntf3
• NCBI Protein Information: neurotrophin-3; HDNF; NT-3; NGF-2; neurotrophic factor; nerve growth factor 2; neurotrophin-3 (HDNF/NT-3)
• UniProt Protein Name: Neurotrophin-3
• UniProt Gene Name: Ntf3
• UniProt Synonym Gene Names: Ntf-3; NT-3; NGF-2
• UniProt Entry Name: NTF3_RAT