Standard Curve (Sample)
(Human TrkB PicoKine ELISA Kit standard curve)
Human TrkB ELISA Kit | NTRK2 elisa kit
Human TrkB PicoKine ELISA Kit
Gene Names
NTRK2; TRKB; trk-B; GP145-TrkB
Reactivity
Human
Synonyms
TrkB; N/A; Human TrkB PicoKine ELISA Kit; GP145 TrkB; GP145-TrkB; NTRK2; Q16620; Trk-B; TrkB tyrosine kinase; BDNF/NT-3 growth factors receptor; neurotrophic receptor tyrosine kinase 2; NTRK2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human TrkB
Sequence Length
477
Assay Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
TMB incubation time
25-30
Immunogen
NSO, C32-H430
Sample Data
Concentration(pg/ml): |0|156|312|625|1250|2500|5000|10000
O.D.: |0.009|0.084|0.149|0.269|0.459|0.818|1.530|2.212
O.D.: |0.009|0.084|0.149|0.269|0.459|0.818|1.530|2.212
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |202|1009|4057|195|887|3332
Standard Deviation: |12.72|54.48|198.73|14.62|54.10|193.25
CV(%): |6.3|5.4|4.9|7.5|6.1|5.8
n: |16|16|16|24|24|24
Mean(pg/ml): |202|1009|4057|195|887|3332
Standard Deviation: |12.72|54.48|198.73|14.62|54.10|193.25
CV(%): |6.3|5.4|4.9|7.5|6.1|5.8
Assay Procedure Step 1
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human TrkB standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates or tissue homogenates to each empty well. It is recommended that each human TrkB standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
(Human TrkB PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human TrkB PicoKine ELISA Kit standard curve)
Related Product Information for NTRK2 elisa kit
For quantitative detection of human TrkB in cell culture supernates, cell lysates and tissue homogenates.
Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3.
Principle: Human TrkB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for TrkB has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: C32-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TrkB amount of sample captured in plate.
Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3.
Principle: Human TrkB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for TrkB has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: C32-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TrkB amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,332 Da
NCBI Official Full Name
BDNF/NT-3 growth factors receptor isoform b
NCBI Official Synonym Full Names
neurotrophic receptor tyrosine kinase 2
NCBI Official Symbol
NTRK2
NCBI Official Synonym Symbols
TRKB; trk-B; GP145-TrkB
NCBI Protein Information
BDNF/NT-3 growth factors receptor
UniProt Protein Name
BDNF/NT-3 growth factors receptor
UniProt Gene Name
NTRK2
UniProt Synonym Gene Names
TRKB; Trk-B
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Product Notes
The Human NTRK2 ntrk2 (Catalog #AAA46762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46762 ELISA Kit recognizes Human NTRK2. It is sometimes possible for the material contained within the vial of "TrkB, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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