Mouse Cross-linked N-telopeptides of collagen type 1 ELISA Kit | NTx elisa kit

Mouse Cross-linked N-telopeptides of collagen type 1 ELISA Kit

Cat. Num. AAA16481

• Reactivity: Mouse
• Synonyms: Cross-linked N-telopeptides of collagen type 1; N/A; Mouse Cross-linked N-telopeptides of collagen type 1 ELISA Kit; NTx elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTX1. No significant cross-reactivity or interference between CTX1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTX1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for NTx elisa kit

Principle of the Assay: CTX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CTX1 antibody and an CTX1-HRP conjugate. The assay sample and buffer are incubated together with CTX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTX1 concentration since CTX1 from samples and CTX1-HRP conjugate compete for the anti-CTX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTX1 from the sample, fewer sites are left to bind CTX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTX1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CTX1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CTX1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for NTx elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4579738
• Molecular Weight: 146,743 Da
• NCBI Official Full Name: NTX, partial
• UniProt Protein Name: NTX
• UniProt Gene Name: ntx
• UniProt Entry Name: Q9QTG7_CBDP