SDS-PAGE
NUAK Family SNF1 Like Kinase 1 Recombinant Protein | NUAK1 recombinant protein
Recombinant NUAK Family SNF1 Like Kinase 1 (NUAK1)
Gene Names
NUAK1; ARK5
Applications
Immunoprecipitation, Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
>90%
Synonyms
NUAK Family SNF1 Like Kinase 1; N/A; Recombinant NUAK Family SNF1 Like Kinase 1 (NUAK1); NUAK1 recombinant protein
Host
E. coli
Purity/Purification
>90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
KRSNSEH RSHSTGFIEG IVSPALPSPF KMEQDLCRTA IPLPSSPEAD MSGKLSLKQS ATMPKKGILK KTQQRESGYY SSPERSESSE LLDSNDVVIS GGLSSPPPDP ARGTSHSLSC RRKGILKHSS RYSDGGTDPA LTRPEMPTLE SLSPPGVPSD GISRSYSRPS SIISDDSVLS SDSFDLLELQ ENRPARQRIR SCVSAENFLQ LQDFETPHNR PRPQYLKRLA DSSFSLLTDMDDVTQVYKKA L
KRSNSEH RSHSTGFIEG IVSPALPSPF KMEQDLCRTA IPLPSSPEAD MSGKLSLKQS ATMPKKGILK KTQQRESGYY SSPERSESSE LLDSNDVVIS GGLSSPPPDP ARGTSHSLSC RRKGILKHSS RYSDGGTDPA LTRPEMPTLE SLSPPGVPSD GISRSYSRPS SIISDDSVLS SDSFDLLELQ ENRPARQRIR SCVSAENFLQ LQDFETPHNR PRPQYLKRLA DSSFSLLTDMDDVTQVYKKA L
Sequence Length
661
Applicable Applications for NUAK1 recombinant protein
IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Source
Prokaryotic expression.
Residues
Lys404~Leu651
Tags
N-terminal His-Tag
Subcellular Location
Nucleus. Cytoplasm.
Traits
Freeze-dried powder
Predicted isoelectric point
7.9
Predicted Molecular Mass
57.2kDa
Accurate Molecular Mass
57kDa as determined by SDS-PAGE reducing conditions
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
NUAK family SNF1-like kinase 1
NCBI Official Synonym Full Names
NUAK family, SNF1-like kinase, 1
NCBI Official Symbol
NUAK1
NCBI Official Synonym Symbols
ARK5
NCBI Protein Information
NUAK family SNF1-like kinase 1
UniProt Protein Name
NUAK family SNF1-like kinase 1
UniProt Gene Name
NUAK1
UniProt Synonym Gene Names
ARK5; KIAA0537; OMPHK1; ARK5
UniProt Entry Name
NUAK1_HUMAN
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Product Notes
The NUAK1 nuak1 (Catalog #AAA144375) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NUAK Family SNF1 Like Kinase 1 can be used in a range of immunoassay formats including, but not limited to, IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the NUAK1 nuak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.KRSNSEH RSHSTGFIEG IVSPALPSPF KMEQDLCRTA IPLPSSPEAD MSGKLSLKQS ATMPKKGILK KTQQRESGYY SSPERSESSE LLDSNDVVIS GGLSSPPPDP ARGTSHSLSC RRKGILKHSS RYSDGGTDPA LTRPEMPTLE SLSPPGVPSD GISRSYSRPS SIISDDSVLS SDSFDLLELQ ENRPARQRIR SCVSAENFLQ LQDFETPHNR PRPQYLKRLA DSSFSLLTDM DDVTQVYKKA L. It is sometimes possible for the material contained within the vial of "NUAK Family SNF1 Like Kinase 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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