Rabbit ARK5 Polyclonal Antibody | anti-NUAK1 antibody

ARK5 Antibody (N-term)

Cat. Num. AAA284550

• Gene Names: NUAK1; ARK5
• Reactivity: Human, Hamster, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ARK5, Antibody; ARK5 Antibody (N-term); NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5; ARK5; Omphalocele kinase 1; NUAK1; KIAA0537; OMPHK1; anti-NUAK1 antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ARK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ARK5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 1-30
• Sequence Length: 661

• Applicable Applications for anti-NUAK1 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: Human
• Function: Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser- 392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair. (PubMed: 25329316).
• Cellular Location: Nucleus. Cytoplasm.
• Tissue Location: Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines. this basic mechanism, protein kinases mediatemost of the signal transduction in eukaryoticcells, regulating cellular metabolism,transcription, cell cycle progression, cytoskeletalrearrangement and cell movement, apoptosis,and differentiation. With more than 500 geneproducts, the protein kinase family is one of thelargest families of proteins in eukaryotes. Thefamily has been classified in 8 major groupsbased on sequence comparison of their tyrosine(PTK) or serine/threonine (STK) kinase catalyticdomains. The STE group (homologs of yeastSterile 7, 11, 20 kinases) consists of 50 kinasesrelated to the mitogen-activated protein kinase(MAPK) cascade families (Ste7/MAP2K,Ste11/MAP3K, and Ste20/MAP4K). MAP kinasecascades, consisting of a MAPK and one or moreupstream regulatory kinases (MAPKKs) havebeen best characterized in the yeast pheromoneresponse pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(The anti-ARK5 Pab is used in Western blot to detect ARK5 in mouse brain tissue lysate.)

Related Product Information for anti-NUAK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-NUAK1 antibody

Cancer; Signal Transduction

References

Suzuki, A., et al., Mol. Cell. Biol. 24(8):3526-3535 (2004).
Suzuki, A., et al., Oncogene 22(40):6177-6182 (2003).
Suzuki, A., et al., J. Biol. Chem. 278(1):48-53 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 7662170
• NCBI GeneID: 9891
• NCBI Accession #: NP_055655.1
• NCBI GenBank Nucleotide #: NM_014840.2
• UniProt Accession #: O60285; Q96KA8; A7MD39
• NCBI Official Full Name: NUAK family SNF1-like kinase 1
• NCBI Official Synonym Full Names: NUAK family, SNF1-like kinase, 1
• NCBI Official Symbol: NUAK1
• NCBI Official Synonym Symbols: ARK5
• NCBI Protein Information: NUAK family SNF1-like kinase 1
• UniProt Protein Name: NUAK family SNF1-like kinase 1
• UniProt Gene Name: NUAK1
• UniProt Synonym Gene Names: ARK5; KIAA0537; OMPHK1; ARK5
• UniProt Entry Name: NUAK1_HUMAN