Standard Curve (Sample)
Human Nesfatin-1 ELISA Kit | NUCB2 elisa kit
Human Nesfatin-1 PicoKine ELISA Kit
Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human
Synonyms
Nesfatin-1; N/A; Human Nesfatin-1 PicoKine ELISA Kit; Nucleobindin-2; nucleobindin 2; DNA binding protein NEFA; DNA-binding protein NEFA; Gastric cancer antigen Zg4; NEFA; Nefastin 1; Nefastin-1; Nefastin1; Nucb2; NUCB2_HUMAN; Nucleobinding 2; Prepronefastin; Prepronesfatin; NUCB2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human NUCB2
Sequence Length
420
Samples
Cell culture supernatants and serum
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NUCB2 elisa kit
Background: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
Principle of the Assay: The Picokine™ Human NUCB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human NUCB2 with a 96-well strip plate that is pre-coated with antibody specific for NUCB2. The detection antibody is a biotinylated antibody specific for NUCB2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human NUCB2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: V25-L106. The kit is analytically validated with ready-to-use reagents. To measure Human NUCB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human NUCB2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human NUCB2 in the sample.
Principle of the Assay: The Picokine™ Human NUCB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human NUCB2 with a 96-well strip plate that is pre-coated with antibody specific for NUCB2. The detection antibody is a biotinylated antibody specific for NUCB2. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human NUCB2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: V25-L106. The kit is analytically validated with ready-to-use reagents. To measure Human NUCB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human NUCB2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human NUCB2 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,201 Da
NCBI Official Full Name
Nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2; nesfatin 1; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109
UniProt Protein Name
Nucleobindin-2
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
UniProt Entry Name
NUCB2_HUMAN
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Product Notes
The Human NUCB2 nucb2 (Catalog #AAA45906) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45906 ELISA Kit recognizes Human NUCB2. It is sometimes possible for the material contained within the vial of "Nesfatin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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