Protease 7 (ompT) Recombinant Protein | ompT recombinant protein

Recombinant Escherichia coli Protease 7 (ompT)

Cat. Num. AAA18640

• Gene Names: ompT; ECK0557; JW0554
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protease 7 (ompT); N/A; Recombinant Escherichia coli Protease 7 (ompT); Omptin; Outer membrane protein 3B; Protease A; Protease VII; ompT recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 21-317aa; Full-Length of the Mature Protein
• Sequence: STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for ompT recombinant protein

Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.

References

Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.Grodberg J., Lundrigan M.D., Toledo D.L., Mangel W.F., Dunn J.J.Nucleic Acids Res. 16:1209-1209(1988) Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues identity of protease VII and OmpT.Sugimura K., Nisihihara T.J. Bacteriol. 170:5625-5632(1988) Sequence of minutes 4-25 of Escherichia coli.Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A.Electrophoresis 19:837-844(1998) Identification of OmpT as the protease that hydrolyzes the antimicrobial peptide protamine before it enters growing cells of Escherichia coli.Stumpe S., Schmid R., Stephens D.L., Georgiou G., Bakker E.P.J. Bacteriol. 180:4002-4006(1998) In vitro folding, purification and characterization of Escherichia coli outer membrane protease ompT.Kramer R.A., Zandwijken D., Egmond M.R., Dekker N.Eur. J. Biochem. 267:885-893(2000) Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site.Kramer R.A., Vandeputte-Rutten L., de Roon G.J., Gros P., Dekker N., Egmond M.R.FEBS Lett. 505:426-430(2001) Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site.Vandeputte-Rutten L., Kramer R.A., Kroon J., Dekker N., Egmond M.R., Gros P.EMBO J. 20:5033-5039(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 16128548
• NCBI GeneID: 945185
• NCBI Accession #: NP_415097.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P09169
• Molecular Weight: 37.5 kDa
• NCBI Official Full Name: DLP12 prophage; outer membrane protease VII; outer membrane protein 3b
• NCBI Official Symbol: ompT
• NCBI Official Synonym Symbols: ECK0557; JW0554
• NCBI Protein Information: DLP12 prophage; outer membrane protease VII; outer membrane protein 3b
• UniProt Protein Name: Protease 7
• UniProt Gene Name: ompT
• UniProt Entry Name: OMPT_ECOLI