Human phosphorylated Tar DNA Binding Protein 43kDa ELISA Kit | P-TDP43 elisa kit

Human phosphorylated Tar DNA Binding Protein 43kDa ELISA Kit

Cat. Num. AAA27398

• Reactivity: Human
• Synonyms: phosphorylated Tar DNA Binding Protein 43kDa; N/A; Human phosphorylated Tar DNA Binding Protein 43kDa ELISA Kit; P-TDP43 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PTDP43. No significant cross-reactivity or interference between PTDP43 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTDP43 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

Standard Curve (Sample)

Related Product Information for P-TDP43 elisa kit

Principle of the Assay: PTDP43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTDP43 antibody and an PTDP43-HRP conjugate. The assay sample and buffer are incubated together with PTDP43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTDP43 concentration since PTDP43 from samples and PTDP43-HRP conjugate compete for the anti-PTDP43 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTDP43 from the sample, fewer sites are left to bind PTDP43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTDP43 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PTDP43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTDP43. This ELISA kit for research use only, not for therapeutic or diagnostic applications!