P2RX7 Recombinant Protein | hFc recombinant protein

Human P2RX7 Protein, hFc Tag

Cat. Num. AAA11710

• Gene Names: P2RX7; P2X7
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: P2RX7; N/A; Human P2RX7 Protein, hFc Tag; P2X7; hFc recombinant protein

• Host: HEK293
• Purity/Purification: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

• Species: Human
• Tag: N-Human Fc tag
• Visual Inspection: Test: Via Label; Specification: Label aligned and complete; Result: Pass; ; Test: Bottle Sealing (manual check); Specification: Sealed tight; Result: Pass
• Quantity: Test: BCA Method; Specification: > Specified quantity; Results: Pass
• Bioactivity: Test: Elisa OD450 value (0.2 ug/well); Specification: Bind to anti-DYKDDDDK monoclonal antibody; Result: Pass
• Other Properties: Test: pH; Specification: 7.4±0.5; Result: Pass
• Target: P2RX7
• Molecular Characterization: hFc(Glu99-Ala330) P2RX7(Ser47-Val334)
• Reconstitution: 1) Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5 min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.; 2) Dissolved lyophilized protein sample in sterile and low endotoxin water based on the recommended volume 37.45 ul; 3) Alter adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
• Preparation and Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

SDS-PAGE

(Figure 1. Human P2RX7 Protein, hFc Tag on SDS-PAGE under reducing condition.)

Related Product Information for hFc recombinant protein

Background: The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

NCBI and Uniprot Product Information

• NCBI GI #: 300068987
• NCBI GeneID: 5027
• NCBI Accession #: NP_002553.3
• NCBI GenBank Nucleotide #: NM_002562.5
• UniProt Accession #: Q99572; O14991; Q4VKH8; Q4VKH9; Q4VKI0; Q4VKI1; Q4VKI2; A8K2Z0; E7EMK6; F5H6P2; F5H7E8; F8W951
• Molecular Weight: The protein has a predicted molecular mass of 59.2 kDa after removal of the signal peptide. The apparent molecular mass of hFc-P2RX7 is approximately 55-70 kDa due to glycosylation
• NCBI Official Full Name: P2X purinoceptor 7
• NCBI Official Synonym Full Names: purinergic receptor P2X, ligand-gated ion channel, 7
• NCBI Official Symbol: P2RX7
• NCBI Official Synonym Symbols: P2X7
• NCBI Protein Information: P2X purinoceptor 7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• UniProt Protein Name: P2X purinoceptor 7
• UniProt Gene Name: P2RX7
• UniProt Synonym Gene Names: P2X7
• UniProt Entry Name: P2RX7_HUMAN