Human programmed cell death 1 ELISA Kit | PDCD1 elisa kit
Human Programmed Death 1, PD-1 ELISA Kit
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Human
Synonyms
programmed cell death 1; N/A; Human Programmed Death 1, PD-1 ELISA Kit; Human Programmed Death 1 (PD-1) ELISA KIT; CD279; PD1; SLEB2; hPD-1; hPD-l; ; PDCD1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human PD-1. No significant cross-reactivity or interference between human PD-1 and analogues was observed.
Sequence Length
288
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for PDCD1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PD-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PD-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PD-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PD-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,647 Da
NCBI Official Full Name
programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
NCBI Protein Information
programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
UniProt Entry Name
PDCD1_HUMAN
