PD-1 Recombinant Protein | PDCD1 recombinant protein

PD-1 Protein, Mouse, Recombinant (ECD, His Tag)

Cat. Num. AAA258130

• Gene Names: Pdcd1; PD-1; Pdc1; Ly101
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC
• Synonyms: PD-1; N/A; PD-1 Protein, Mouse, Recombinant (ECD, His Tag); Mouse PD1/PDCD1 Protein (ECD,His Tag); Ly101 Protein, Mouse; PD-1 Protein, Mouse; Pdc1 Protein, Mouse; programmed cell death 1; PDCD1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5% trehalose, 5% mannitol
• Sequence: Met1-Gln167

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Gln 167) of mouse PDCD1 (NP_032824.1) precursor was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/ml. Concentration is measured by UV
• Bio Activity: Measured by its ability to bind PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag) (MBS8118825) in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C.; Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.; Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for PDCD1 recombinant protein

Background: Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gamma by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

Product Categories/Family for PDCD1 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.

NCBI and Uniprot Product Information

• NCBI GI #: 6679239
• NCBI GeneID: 18566
• NCBI Accession #: NP_032824.1
• NCBI GenBank Nucleotide #: NM_008798.2
• UniProt Accession #: Q02242
• Molecular Weight: The secreted recombinant mouse PDCD1 consists of 154 amino acids and has a predicted molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of mouse PDCD1 is approximately 40-45 kDa due to glycosylation.
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: Pdcd1
• NCBI Official Synonym Symbols: PD-1; Pdc1; Ly101
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: Pdcd1
• UniProt Synonym Gene Names: Pd1; Protein PD-1; mPD-1
• UniProt Entry Name: PDCD1_MOUSE