Application Data
PDE2A recombinant protein
PDE2A, Active
Gene Names
PDE2A; PDE2A1; PED2A4; cGSPDE; CGS-PDE
Applications
PDE Assay
Synonyms
PDE2A; N/A; PDE2A, Active; PDE2A recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full Length
Sequence Length
4329
Applicable Applications for PDE2A recombinant protein
PDE Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PDE2A recombinant protein
Description: Recombinant full-length human PDE2A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE2A is a member of the cyclic nucleotide phosphodiesterase family. PDE2A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. TNFalpha stimulation of cells leads to an increase in PDE2A expression in a p38 mitogen-activated protein kinase (MAPK)-dependent manner (1). TNFalpha-mediated up-regulation of PDE2A may destabilize endothelial barrier function in sepsis. In addition, selective PDE2A up-regulation sensitizesendothelial cells toward the permeability-increasing agent thrombin (2). Furthermore, PDE2A inhibition is effective in preventing thrombin-induced lung edema.
Scientific Background: PDE2A is a member of the cyclic nucleotide phosphodiesterase family. PDE2A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. TNFalpha stimulation of cells leads to an increase in PDE2A expression in a p38 mitogen-activated protein kinase (MAPK)-dependent manner (1). TNFalpha-mediated up-regulation of PDE2A may destabilize endothelial barrier function in sepsis. In addition, selective PDE2A up-regulation sensitizesendothelial cells toward the permeability-increasing agent thrombin (2). Furthermore, PDE2A inhibition is effective in preventing thrombin-induced lung edema.
Product Categories/Family for PDE2A recombinant protein
References
1. Seybold J, et al: Tumor necrosis factor-alpha-dependent expression of phosphodiesterase 2: role in endothelial hyperpermeability. Blood. 2005 May 1;105 (9):3569-76. 2. Diebold I, et al: Phosphodiesterase 2 mediates redox-sensitive endothelial cell proliferation and angiogenesis by thrombin via Rac1 and NADPH oxidase 2. Circ Res. 2009 May 22;104 (10):1169-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~130 kDa
NCBI Official Full Name
Homo sapiens phosphodiesterase 2A (PDE2A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 2A
NCBI Official Symbol
PDE2A
NCBI Official Synonym Symbols
PDE2A1; PED2A4; cGSPDE; CGS-PDE
NCBI Protein Information
cGMP-dependent 3',5'-cyclic phosphodiesterase
UniProt Protein Name
cGMP-dependent 3',5'-cyclic phosphodiesterase
UniProt Gene Name
PDE2A
UniProt Synonym Gene Names
CGS-PDE; cGSPDE
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Product Notes
The PDE2A pde2a (Catalog #AAA73097) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length. AAA Biotech's PDE2A can be used in a range of immunoassay formats including, but not limited to, PDE Assay. Researchers should empirically determine the suitability of the PDE2A pde2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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