PDGFR beta Recombinant Protein | CD140b recombinant protein
Rat PDGFR beta Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
PDGFR beta; N/A; Rat PDGFR beta Recombinant; Rat CD140b Recombinant (PDGF Receptor beta) ; cd-140 PDGFRB; CD140B; MGC74795; PDGFR2; Rhe-PDGFRB PDGF Receptor alpha; pdgf-r; CD140b recombinant protein
Host
N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant PDGFR beta is supplied as a 0.2 um filtered PBS solution, pH7.2.
Sequence Length
1097
Domain
Ig-like C2
Source
HEk293 Cells
Source Note
Optimized DNA sequence encoding extracellular domain of rat PDGFR beta ncluding a C-terminal 6His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Weight Note
Recombinant rat PDGF-R beta is a monomer protein consisting of 510 amino acid residue subunits, due to glycosylation migrates as an approximately 100 kDa protein on SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree to 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree to 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for CD140b recombinant protein
The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (PDGFA, PDGFB, PDGFC, and PDGFD. Ligand binding induces receptor dimerization, enabling autophosphorylation of specific tyrosine residues and subsequent recruitment of a variety of signal transduction molecules. PDGFR regulates normal cellular growth and differentiation, and expression of activated PDGFR promotes oncogenic transformation, suggesting that activating mutations or gene rearrangements could play a role in human tumorigenesis. Numerous in vitro and in vivo studies showed that inhibition of PDGFRA signaling disrupts cancer cell survival in the subset of GISTs with activating PDGFRA mutations.
Product Categories/Family for CD140b recombinant protein
References
• hydrophobic matching controls the tilt and stability of the dimeric platelet-derived growth factor receptor (pdgfr) β transmembrane segment .J. Biol. Chem., Jul 2012; 287: 26178 - 26186.
• pdgfrβ expression and function in fibroblasts derived from pluripotent cells is linked to dna demethylation .J. Cell Sci., May 2012; 125: 2276 - 2287.
• pdgf-induced migration of vascular smooth muscle cells is inhibited by heme oxygenase-1 via vegfr2 upregulation and subsequent assembly of inactive vegfr2/pdgfrβ heterodimers .Arterioscler Thromb Vasc Biol, May 2012; 32: 1289 - 1298.
• a viable mouse pdgfr-beta mutant exhibits cns-restricted pericyte deficiency and blood brain barrier defects .Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 2558.
• the leukemia-associated fusion protein tel-platelet-derived growth factor receptor β (tel-pdgfrβ) inhibits transcriptional repression of ptpn13 gene by interferon consensus sequence binding protein (icsbp) .J. Biol. Chem., Mar 2012; 287: 8110 - 8125.
• pdgfrβ expression and function in fibroblasts derived from pluripotent cells is linked to dna demethylation .J. Cell Sci., May 2012; 125: 2276 - 2287.
• pdgf-induced migration of vascular smooth muscle cells is inhibited by heme oxygenase-1 via vegfr2 upregulation and subsequent assembly of inactive vegfr2/pdgfrβ heterodimers .Arterioscler Thromb Vasc Biol, May 2012; 32: 1289 - 1298.
• a viable mouse pdgfr-beta mutant exhibits cns-restricted pericyte deficiency and blood brain barrier defects .Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 2558.
• the leukemia-associated fusion protein tel-platelet-derived growth factor receptor β (tel-pdgfrβ) inhibits transcriptional repression of ptpn13 gene by interferon consensus sequence binding protein (icsbp) .J. Biol. Chem., Mar 2012; 287: 8110 - 8125.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
platelet-derived growth factor receptor beta
UniProt Protein Name
Platelet-derived growth factor receptor beta
UniProt Gene Name
Pdgfrb
UniProt Synonym Gene Names
Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1
UniProt Entry Name
PGFRB_RAT
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Product Notes
The CD140b pdgfrb (Catalog #AAA76115) is a Recombinant Protein produced from N/A (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDGFR beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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