Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
PDK1 recombinant protein
Recombinant PDK1 protein, GST-Tag
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Synonyms
PDK1; N/A; Recombinant PDK1 protein, GST-Tag; Pyruvate Dehydrogenase Kinase 1; PDK1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant PDK1 protein, GST-Tag is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant PDK1 protein, GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
SDS-PAGE
(Recombinant PDK1 protein, GST-Tag 10% SDS-PAGE Coomassie staining MW: 89.8 kDa Purity: >70%)
SDS-PAGE
(Recombinant PDK1 protein, GST-Tag 10% SDS-PAGE Coomassie staining MW: 89.8 kDa Purity: >70%)
Related Product Information for PDK1 recombinant protein
Short Description: Recombinant PDK1 protein, GST-Tag was expressed in a baculovirus expression system as the full-length protein (accession number NP_002604.1) with an N-terminal GST tag. The molecular weight of the protein is 89.8 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: PDK1 (Pyruvate Dehydrogenase Kinase 1), also known as PDHK1, is a serine/threonine kinase. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. PDK1 plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. Its activity can be enhanced by binding to the pyruvate dehydrogenase subunit DLAT, inhibited by AZD7545 (this compound interferes with DLAT binding and thereby inhibits kinase activity). Its activity can also be inhibited by dichloroacetate and radicicol.
Background: PDK1 (Pyruvate Dehydrogenase Kinase 1), also known as PDHK1, is a serine/threonine kinase. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. PDK1 plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. Its activity can be enhanced by binding to the pyruvate dehydrogenase subunit DLAT, inhibited by AZD7545 (this compound interferes with DLAT binding and thereby inhibits kinase activity). Its activity can also be inhibited by dichloroacetate and radicicol.
Product Categories/Family for PDK1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,838 Da
NCBI Official Full Name
3-phosphoinositide-dependent protein kinase 1 isoform 1
NCBI Official Synonym Full Names
3-phosphoinositide dependent protein kinase 1
NCBI Official Symbol
PDPK1
NCBI Official Synonym Symbols
PDK1; PDPK2; PDPK2P; PRO0461
NCBI Protein Information
3-phosphoinositide-dependent protein kinase 1
UniProt Protein Name
3-phosphoinositide-dependent protein kinase 1
UniProt Gene Name
PDPK1
UniProt Synonym Gene Names
PDK1; hPDK1
UniProt Entry Name
PDPK1_HUMAN
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Product Notes
The PDK1 pdpk1 (Catalog #AAA60178) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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