phospho-LIMK1 (Thr-508) Peptide | LIMK1 peptide
phospho-LIMK1 (Thr-508) Peptide
Gene Names
LIMK1; LIMK; LIMK-1
Applications
ELISA
Synonyms
phospho-LIMK1 (Thr-508) Peptide; N/A; LIMK1 peptide
Specificity
The peptide is specifically recognized by LIMK1 (Thr-508) antibody (LP1891) in ELISA, and has been shownto block the reactivity of LP1891 in Western blot and immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Applicable Applications for LIMK1 peptide
Blocking, ELISA
Seuqnece
Peptide Sequence: Phospho-LIMK1 (Thr-508) synthetic peptide corresponding to amino acids surrounding threonine 508 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Thr-505 in human LIMK2.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Related Product Information for LIMK1 peptide
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their aminoterminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.
References
• Okano, I. et al. (1995) J. Biol. Chem. 270:31321.
• Edwards, D. C. et al. (1999) Nat. Cell Biol. 1:253.
• Kobayashi, M. et al. (2006) EMBOJ 25:713.
• Edwards, D. C. et al. (1999) Nat. Cell Biol. 1:253.
• Kobayashi, M. et al. (2006) EMBOJ 25:713.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
LIM domain kinase 1 isoform 2
NCBI Official Synonym Full Names
LIM domain kinase 1
NCBI Official Symbol
LIMK1
NCBI Official Synonym Symbols
LIMK; LIMK-1
NCBI Protein Information
LIM domain kinase 1; OTTHUMP00000160532; OTTHUMP00000211913; LIM motif-containing protein kinase
UniProt Protein Name
LIM domain kinase 1
UniProt Gene Name
LIMK1
UniProt Synonym Gene Names
LIMK
UniProt Entry Name
LIMK1_HUMAN
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Product Notes
The LIMK1 limk1 (Catalog #AAA71542) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's phospho-LIMK1 (Thr-508) Peptide can be used in a range of immunoassay formats including, but not limited to, Blocking, ELISA. Researchers should empirically determine the suitability of the LIMK1 limk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "phospho-LIMK1 (Thr-508) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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