Application Data
(The purity of PIP5K1A was determined to be >85% by densitometry, approx. MW 86 kDa)
PIP5K1A recombinant protein
PIP5K1A, Active
Purity
>85%
Synonyms
PIP5K1A; N/A; PIP5K1A, Active; PIP5K1A recombinant protein
Purity/Purification
>85%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Concentration
0.1 ug/ul (varies by lot)
Sequence Length
3628
Specific Activity
4,000 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -70 degree C. Stable for one year at -70 degree C upon receipt.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of PIP5K1A was determined to be >85% by densitometry, approx. MW 86 kDa)
Application Data
(The purity of PIP5K1A was determined to be >85% by densitometry, approx. MW 86 kDa)
Application Data
(The specific activity of PIP5K1A was determined to be 4,000 nmol/min/mg as per activity assay protocol.)
Application Data
(The specific activity of PIP5K1A was determined to be 4,000 nmol/min/mg as per activity assay protocol.)
Related Product Information for PIP5K1A recombinant protein
Description: Recombinant full-length human PIP5K1A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells (1). Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'dishevelled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP (2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes (2). Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP (2) production and enhances viral replication in primary lymphocytes.
Scientific Background: PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells (1). Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'dishevelled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP (2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes (2). Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP (2) production and enhances viral replication in primary lymphocytes.
Product Categories/Family for PIP5K1A recombinant protein
References
1. Pan, W. et al: Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation. Science 321: 1350-1353, 2008. 2. Barrero-Villar M, PI4P5-kinase Ialpha is required for efficient HIV-1 entry and infection of T cells. J Immunol. 2008 Nov 15;181 (10):6882-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
NCBI Official Symbol
PIP5K1A
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Gene Name
PIP5K1A
UniProt Synonym Gene Names
PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha
Similar Products
Product Notes
The PIP5K1A pip5k1a (Catalog #AAA14257) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PIP5K1A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.