WB (Western Blot)
(Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human PIP5K1A Polyclonal Antibody | anti-PIP5K1A antibody
PIP5K1A Antibody (N-term)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PIP5K1A, Antibody; PIP5K1A Antibody (N-term); Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; PIP5KIalpha; PIP5K1A; anti-PIP5K1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PIP5K1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-50 amino acids from the N-terminal region of human PIP5K1A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
19-50
Sequence Length
522
Applicable Applications for anti-PIP5K1A antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of PIP5K1A (arrow) using rabbit polyclonal hPIP5K1A-R34. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIP5K1A gene.)
WB (Western Blot)
(Western blot analysis of PIP5K1A (arrow) using rabbit polyclonal hPIP5K1A-R34. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIP5K1A gene.)
Related Product Information for anti-PIP5K1A antibody
Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.
Product Categories/Family for anti-PIP5K1A antibody
References
Doughman, R.L., et al., J. Biol. Chem. 278(25):23036-23045 (2003).
Loijens, J.C., et al., J. Biol. Chem. 271(51):32937-32943 (1996).
Xie, Y., et al., Cytogenet. Cell Genet. 88 (3-4), 197-199 (2000).
Loijens, J.C., et al., J. Biol. Chem. 271(51):32937-32943 (1996).
Xie, Y., et al., Cytogenet. Cell Genet. 88 (3-4), 197-199 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62633
NCBI Official Full Name
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 3
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
NCBI Official Symbol
PIP5K1A
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Gene Name
PIP5K1A
UniProt Synonym Gene Names
PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha
UniProt Entry Name
PI51A_HUMAN
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Product Notes
The PIP5K1A pip5k1a (Catalog #AAA289759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 19-50. The PIP5K1A Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIP5K1A can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PIP5K1A pip5k1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIP5K1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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