Application Data
(The purity of VLK was determined to be ~75% by densitometryApprox MW 69 kDa)
VLK recombinant protein
VLK recombinant protein
Gene Names
PKDCC; Vlk; SGK493
Purity
~75%
Synonyms
VLK; N/A; VLK recombinant protein; SGK493; PKDCC
Host
Sf9 insect cells
Purity/Purification
~75%
Form/Format
Recombinant protein stored in 50 mM Tris-HCI, pH 7.5, 150 mM NaCI, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Concentration
0.05 ug/ul (varies by lot)
Sequence Length
2517
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant human VLK (129-end) was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of VLK was determined to be ~75% by densitometryApprox MW 69 kDa)
Application Data
(The purity of VLK was determined to be ~75% by densitometryApprox MW 69 kDa)
Related Product Information for VLK recombinant protein
Recombinant human VLK (129-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: VLK or vertebrate lonesome kinase is a protein kinase which is first expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, and later its expression is confined to E-cadherin-negative mesenchyme. VLK regulates the rate of protein export from the Golgi and thereby plays an important role in the formation of functional stroma by mesenchymal cells (1). Targeted disruption of VLK leads to a defect in lung development and delayed ossification of endochondral bone. VLK deficient mice display neonatal lethality due to respiratory failure, with a suckling defect arising from a cleft palate. VLK is required for the appropriate timing of flat proliferative chondrocyte differentiation (2).
Scientific Background: VLK or vertebrate lonesome kinase is a protein kinase which is first expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, and later its expression is confined to E-cadherin-negative mesenchyme. VLK regulates the rate of protein export from the Golgi and thereby plays an important role in the formation of functional stroma by mesenchymal cells (1). Targeted disruption of VLK leads to a defect in lung development and delayed ossification of endochondral bone. VLK deficient mice display neonatal lethality due to respiratory failure, with a suckling defect arising from a cleft palate. VLK is required for the appropriate timing of flat proliferative chondrocyte differentiation (2).
Product Categories/Family for VLK recombinant protein
References
1. Kinoshita. et.al: The novel protein kinase Vlk is essential for stromal function of mesenchymal cells.Development 136: 2069-2079, 2009.
2. Imuta, Y. et al: Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124). Dev Dyn. 2009 Jan;238(1):210-22.
2. Imuta, Y. et al: Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124). Dev Dyn. 2009 Jan;238(1):210-22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~69 kDa
NCBI Official Full Name
Homo sapiens protein kinase domain containing, cytoplasmic (PKDCC), mRNA
NCBI Official Synonym Full Names
protein kinase domain containing, cytoplasmic
NCBI Official Symbol
PKDCC
NCBI Official Synonym Symbols
Vlk; SGK493
NCBI Protein Information
protein kinase domain-containing protein, cytoplasmic; sugen kinase 493; vertebrate lonesome kinase; protein kinase-like protein SgK493; protein kinase domain containing, cytoplasmic homolog
UniProt Protein Name
Protein kinase domain-containing protein, cytoplasmic
UniProt Gene Name
PKDCC
UniProt Synonym Gene Names
SGK493; VLK
UniProt Entry Name
PKDCC_HUMAN
Similar Products
Product Notes
The VLK pkdcc (Catalog #AAA72915) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human VLK (129-end) was expressed by baculovirus in Sf9 insect cells. It is sometimes possible for the material contained within the vial of "VLK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.