PLA2G2A recombinant protein

PLA2G2A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257967

• Gene Names: PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Purity: >97% as determined by SDS-PAGE; >90% as determined by SEC-HPLC
• Synonyms: PLA2G2A; N/A; PLA2G2A Protein, Human, Recombinant (His Tag); Human PLA2G2A/PLA2B Protein (His Tag); MOM1 Protein, Human; PLA2 Protein, Human; PLA2B Protein, Human; PLA2L Protein, Human; PLA2S Protein, Human; PLAS1 Protein, Human; sPLA2 Protein, Human; phospholipase A2, group IIA (platelets, synovial fluid); PLA2G2A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE; >90% as determined by SEC-HPLC
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence: Met1-Cys144

• Source: Human; HEK293 Cells-derived Human PLA2G2A / PLA2B Protein (His Tag)
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PLA2G2A (NP_000291.1)(Met 1-Cys 144) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: Reconstituted with sterile deionized water to 0.2 mg/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-PAGE

Related Product Information for PLA2G2A recombinant protein

Background: Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein that belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.

References

Praml,C. et al., 1998, Oncogene. 17 (15):2009-12. Fijneman,R.J. et al., 2008,Front Biosci 13 :4144-74. Fijneman,R.J. et al., 2009, Cell Oncol 31 (5):345-56.

NCBI and Uniprot Product Information

• NCBI GI #: 4505849
• NCBI GeneID: 5320
• NCBI Accession #: NP_000291.1
• NCBI GenBank Nucleotide #: NM_000300.3
• UniProt Accession #: P14555; Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
• Molecular Weight: Predicted Molecular Mass: 15.36kDa; The recombinant human PLA2G2A consists of 135 amino acids and predictes a molecular mass of 15.4 kDa. In SDS-PAGE under reducing conditions, rh PLA2G2A migrates as an approximately 19 kDa band due to glycosylation.
• NCBI Official Full Name: phospholipase A2, membrane associated
• NCBI Official Synonym Full Names: phospholipase A2, group IIA (platelets, synovial fluid)
• NCBI Official Symbol: PLA2G2A
• NCBI Official Synonym Symbols: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• NCBI Protein Information: phospholipase A2, membrane associated; GIIC sPLA2; NPS-PLA2; group IIA phospholipase A2; non-pancreatic secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A
• UniProt Protein Name: Phospholipase A2, membrane associated
• UniProt Gene Name: PLA2G2A
• UniProt Synonym Gene Names: PLA2B; PLA2L; RASF-A; NPS-PLA2
• UniProt Entry Name: PA2GA_HUMAN