SDS-PAGE
Plasminogen Activator, Tissue (tPA) Recombinant Protein | tPA recombinant protein
Recombinant Plasminogen Activator, Tissue (tPA)
Gene Names
Plat; tPA; AU020998; AW212668; D8Ertd2e
Applications
Western Blot, SDS-Page
Purity
> 97%
Synonyms
Plasminogen Activator, Tissue (tPA); N/A; Recombinant Plasminogen Activator, Tissue (tPA); tPA recombinant protein
Host
E Coli
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl, 5%Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
ER FPPNHLKVVL GRTYRVVPGE EEQTFEIEKY IVHEEFDDDT YDNDIALLQL RSQSKQCAQE SSSVGTACLP DPNLQLPDWT ECELSGYGKH EASSPFFSDR LKEAHVRLYP SSRCTSQHLF NKTVTNNMLC AGDTRSGGNQ DLHDACQGDS GGPLVCMINK QMTLTGIISW GL
Applicable Applications for tPA recombinant protein
WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
Organism
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
Glu359~Leu532
Tags
N-terminal His-Tag
Subcellular Location
Secreted, Extracellular matrix
Traits
Freeze-dried powder
Predicted isoelectric point
5.5
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 20.7kDa
Accurate: 23kDa as determined by SDS-PAGE reducing conditions.
Accurate: 23kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
tissue-type plasminogen activator preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
Plat
NCBI Official Synonym Symbols
tPA; AU020998; AW212668; D8Ertd2e
NCBI Protein Information
tissue-type plasminogen activator; t-plasminogen activator
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Gene Name
Plat
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_MOUSE
Similar Products
Product Notes
The tPA plat (Catalog #AAA134289) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Plasminogen Activator, Tissue (tPA) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), SDS-PAGE, Immunogen, Positive Control. Researchers should empirically determine the suitability of the tPA plat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ER FPPNHLKVVL GRTYRVVPGE EEQTFEIEKY IVHEEFDDDT YDNDIALLQL RSQSKQCAQE SSSVGTACLP DPNLQLPDWT ECELSGYGKH EASSPFFSDR LKEAHVRLYP SSRCTSQHLF NKTVTNNMLC AGDTRSGGNQ DLHDACQGDS GGPLVCMINK QMTLTGIISW GL. It is sometimes possible for the material contained within the vial of "Plasminogen Activator, Tissue (tPA), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.